Masticatory lubrication. The role of carbohydrate in the lubricating property of a salivary glycoprotein-albumin complex

M. N. Hatton; R. E. Loomis; M. J. Levine; L. A. Tabak

doi:10.1042/bj2300817

Masticatory lubrication. The role of carbohydrate in the lubricating property of a salivary glycoprotein-albumin complex

M. N. Hatton
, R. E. Loomis
, M. J. Levine
, L. A. Tabak

University at Buffalo

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

125 Scopus citations

Abstract

We report for the first time a masticatory-lubrication assay system to assess the lubricating properties of salivary constituents. The lubricating ability of the proline-rich glycoprotein (PRG) of parotid saliva was enhanced by human serum albumin. The interactive effect of albumin was abolished by chemically deglycosylating the glycoprotein. Fluorescence spectroscopy with a hydrophobic probe verified the existence of a PRG-albumin complex and demonstrated that deglycosylation of the PRG altered the nature of its interaction with albumin.

Original language	English
Pages (from-to)	817-820
Number of pages	4
Journal	Biochemical Journal
Volume	230
Issue number	3
DOIs	https://doi.org/10.1042/bj2300817
State	Published - 1985

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abstract = "We report for the first time a masticatory-lubrication assay system to assess the lubricating properties of salivary constituents. The lubricating ability of the proline-rich glycoprotein (PRG) of parotid saliva was enhanced by human serum albumin. The interactive effect of albumin was abolished by chemically deglycosylating the glycoprotein. Fluorescence spectroscopy with a hydrophobic probe verified the existence of a PRG-albumin complex and demonstrated that deglycosylation of the PRG altered the nature of its interaction with albumin.",

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AU - Loomis, R. E.

AU - Levine, M. J.

AU - Tabak, L. A.

PY - 1985

Y1 - 1985

N2 - We report for the first time a masticatory-lubrication assay system to assess the lubricating properties of salivary constituents. The lubricating ability of the proline-rich glycoprotein (PRG) of parotid saliva was enhanced by human serum albumin. The interactive effect of albumin was abolished by chemically deglycosylating the glycoprotein. Fluorescence spectroscopy with a hydrophobic probe verified the existence of a PRG-albumin complex and demonstrated that deglycosylation of the PRG altered the nature of its interaction with albumin.

AB - We report for the first time a masticatory-lubrication assay system to assess the lubricating properties of salivary constituents. The lubricating ability of the proline-rich glycoprotein (PRG) of parotid saliva was enhanced by human serum albumin. The interactive effect of albumin was abolished by chemically deglycosylating the glycoprotein. Fluorescence spectroscopy with a hydrophobic probe verified the existence of a PRG-albumin complex and demonstrated that deglycosylation of the PRG altered the nature of its interaction with albumin.

UR - https://www.scopus.com/pages/publications/0022376312

U2 - 10.1042/bj2300817

DO - 10.1042/bj2300817

M3 - Article

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JO - Biochemical Journal

JF - Biochemical Journal

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ER -

Masticatory lubrication. The role of carbohydrate in the lubricating property of a salivary glycoprotein-albumin complex

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