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Measuring the Reversible Oxidation of Protein Tyrosine Phosphatases Using a Modified Cysteinyl-Labeling Assay

  • SUNY Polytechnic Institute

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations

Abstract

The modified cysteinyl-labeling assay enables the labeling, enrichment, and detection of all members of the protein tyrosine phosphatase (PTP) superfamily that become reversibly oxidized in cells to facilitate phosphorylation-dependent signaling. In this chapter, we describe the method in detail and highlight the pitfalls of avoiding post-lysis oxidation of PTPs to measure the dynamic and transient oxidation and reduction of PTPs in cell signaling.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages223-237
Number of pages15
DOIs
StatePublished - 2024

Publication series

NameMethods in Molecular Biology
Volume2743

Keywords

  • Biotin labeling
  • Protein tyrosine phosphatases
  • Redox signaling
  • Reversible oxidation
  • Sulfhydryl reactive probes

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