Metabolism of 2,3,7,8-tetrachlorodibenzofuran by rainbow trout (Oncorhynchus mykiss)

A. Ruth Steward; Robert Maslanka; Subodh Kumar; Harish C. Sikka

doi:10.1016/0045-6535(92)90132-B

Metabolism of 2,3,7,8-tetrachlorodibenzofuran by rainbow trout (Oncorhynchus mykiss)

A. Ruth Steward
, Robert Maslanka
, Subodh Kumar
, Harish C. Sikka

Pharmacology and Toxicology

University at Buffalo

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Bile was collected 7 days after treating rainbow trout with 1 ppb [³H]2,3,7,8-tetrachlorodibenzofuran (TCDF). HPLC analysis of methylated extracts of this bile, before and after hydrolysis with β-glucuronidase and arylsulfatase, revealed that the major metabolite was the glucuronide conjugate of 4-OH-2,3,7,8-TCDF, which amounted to 38% of the TCDF-derived radioactivity in bile. In order to examine the inherent capability of trout liver to metabolize this compound under defined conditions, [³H]TCDF was incubated with freshly isolated hepatocytes prepared from rainbow trout pretreated with 3-methylcholanthrene (an inducer of liver monooxygenase enzymes). The maximum (initial) metabolism observed was 20 pmol TCDF equiv/mg dry wt in 30 min. Extraction and HPLC analysis of cells and medium revealed one major metabolite: 4-OH-2,3,7,8-TCDF.

Original language	English
Pages (from-to)	1215-1220
Number of pages	6
Journal	Chemosphere
Volume	25
Issue number	7-10
DOIs	https://doi.org/10.1016/0045-6535(92)90132-B
State	Published - 1992

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@article{9e2bb5b1bfa34b4e965ee5c57f30c557,

title = "Metabolism of 2,3,7,8-tetrachlorodibenzofuran by rainbow trout (Oncorhynchus mykiss)",

abstract = "Bile was collected 7 days after treating rainbow trout with 1 ppb [3H]2,3,7,8-tetrachlorodibenzofuran (TCDF). HPLC analysis of methylated extracts of this bile, before and after hydrolysis with β-glucuronidase and arylsulfatase, revealed that the major metabolite was the glucuronide conjugate of 4-OH-2,3,7,8-TCDF, which amounted to 38\% of the TCDF-derived radioactivity in bile. In order to examine the inherent capability of trout liver to metabolize this compound under defined conditions, [3H]TCDF was incubated with freshly isolated hepatocytes prepared from rainbow trout pretreated with 3-methylcholanthrene (an inducer of liver monooxygenase enzymes). The maximum (initial) metabolism observed was 20 pmol TCDF equiv/mg dry wt in 30 min. Extraction and HPLC analysis of cells and medium revealed one major metabolite: 4-OH-2,3,7,8-TCDF.",

author = "Steward, \{A. Ruth\} and Robert Maslanka and Subodh Kumar and Sikka, \{Harish C.\}",

year = "1992",

doi = "10.1016/0045-6535(92)90132-B",

language = "English",

volume = "25",

pages = "1215--1220",

journal = "Chemosphere",

issn = "0045-6535",

publisher = "Elsevier Ltd",

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TY - JOUR

T1 - Metabolism of 2,3,7,8-tetrachlorodibenzofuran by rainbow trout (Oncorhynchus mykiss)

AU - Steward, A. Ruth

AU - Maslanka, Robert

AU - Kumar, Subodh

AU - Sikka, Harish C.

PY - 1992

Y1 - 1992

N2 - Bile was collected 7 days after treating rainbow trout with 1 ppb [3H]2,3,7,8-tetrachlorodibenzofuran (TCDF). HPLC analysis of methylated extracts of this bile, before and after hydrolysis with β-glucuronidase and arylsulfatase, revealed that the major metabolite was the glucuronide conjugate of 4-OH-2,3,7,8-TCDF, which amounted to 38% of the TCDF-derived radioactivity in bile. In order to examine the inherent capability of trout liver to metabolize this compound under defined conditions, [3H]TCDF was incubated with freshly isolated hepatocytes prepared from rainbow trout pretreated with 3-methylcholanthrene (an inducer of liver monooxygenase enzymes). The maximum (initial) metabolism observed was 20 pmol TCDF equiv/mg dry wt in 30 min. Extraction and HPLC analysis of cells and medium revealed one major metabolite: 4-OH-2,3,7,8-TCDF.

AB - Bile was collected 7 days after treating rainbow trout with 1 ppb [3H]2,3,7,8-tetrachlorodibenzofuran (TCDF). HPLC analysis of methylated extracts of this bile, before and after hydrolysis with β-glucuronidase and arylsulfatase, revealed that the major metabolite was the glucuronide conjugate of 4-OH-2,3,7,8-TCDF, which amounted to 38% of the TCDF-derived radioactivity in bile. In order to examine the inherent capability of trout liver to metabolize this compound under defined conditions, [3H]TCDF was incubated with freshly isolated hepatocytes prepared from rainbow trout pretreated with 3-methylcholanthrene (an inducer of liver monooxygenase enzymes). The maximum (initial) metabolism observed was 20 pmol TCDF equiv/mg dry wt in 30 min. Extraction and HPLC analysis of cells and medium revealed one major metabolite: 4-OH-2,3,7,8-TCDF.

UR - https://www.scopus.com/pages/publications/0027053873

U2 - 10.1016/0045-6535(92)90132-B

DO - 10.1016/0045-6535(92)90132-B

M3 - Article

SN - 0045-6535

VL - 25

SP - 1215

EP - 1220

JO - Chemosphere

JF - Chemosphere

IS - 7-10

ER -

Metabolism of 2,3,7,8-tetrachlorodibenzofuran by rainbow trout (Oncorhynchus mykiss)

Abstract

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