Method to detect functional estrogen receptor expression using estrogen receptor probing compound

Hongbiao Liu; Yi Ding; Rhonda R. Voskuhl

doi:10.1080/15321810500220894

Method to detect functional estrogen receptor expression using estrogen receptor probing compound

Hongbiao Liu
, Yi Ding
, Rhonda R. Voskuhl

Neurology

University at Buffalo

University of California at Los Angeles

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A novel method to detect functional estrogen receptor expression with estrogen receptor probing compound (ERPC) has been established. ERPC is composed of estradiol-BSA-biotin. ERPC can be used to detect functional estrogen receptor expression in both Western blot and immunohistochemistry. ERPC based techniques are non-radioactive, sensitive, relatively inexpensive, and can be used with all species. The use of both ERPC and ERα- or ERβ-specific antibodies provides complementary information in characterizing estrogen receptors' expression and functional binding to ligand.

Original language	English
Pages (from-to)	295-301
Number of pages	7
Journal	Journal of Immunoassay and Immunochemistry
Volume	26
Issue number	4
DOIs	https://doi.org/10.1080/15321810500220894
State	Published - 2005

Keywords

BSA
Biotin
Estradiol
Estrogen receptor
Immunohistochemistry
Western blot

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10.1080/15321810500220894

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title = "Method to detect functional estrogen receptor expression using estrogen receptor probing compound",

abstract = "A novel method to detect functional estrogen receptor expression with estrogen receptor probing compound (ERPC) has been established. ERPC is composed of estradiol-BSA-biotin. ERPC can be used to detect functional estrogen receptor expression in both Western blot and immunohistochemistry. ERPC based techniques are non-radioactive, sensitive, relatively inexpensive, and can be used with all species. The use of both ERPC and ERα- or ERβ-specific antibodies provides complementary information in characterizing estrogen receptors' expression and functional binding to ligand.",

keywords = "BSA, Biotin, Estradiol, Estrogen receptor, Immunohistochemistry, Western blot",

author = "Hongbiao Liu and Yi Ding and Voskuhl, \{Rhonda R.\}",

year = "2005",

doi = "10.1080/15321810500220894",

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pages = "295--301",

journal = "Journal of Immunoassay and Immunochemistry",

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TY - JOUR

T1 - Method to detect functional estrogen receptor expression using estrogen receptor probing compound

AU - Liu, Hongbiao

AU - Ding, Yi

AU - Voskuhl, Rhonda R.

PY - 2005

Y1 - 2005

N2 - A novel method to detect functional estrogen receptor expression with estrogen receptor probing compound (ERPC) has been established. ERPC is composed of estradiol-BSA-biotin. ERPC can be used to detect functional estrogen receptor expression in both Western blot and immunohistochemistry. ERPC based techniques are non-radioactive, sensitive, relatively inexpensive, and can be used with all species. The use of both ERPC and ERα- or ERβ-specific antibodies provides complementary information in characterizing estrogen receptors' expression and functional binding to ligand.

AB - A novel method to detect functional estrogen receptor expression with estrogen receptor probing compound (ERPC) has been established. ERPC is composed of estradiol-BSA-biotin. ERPC can be used to detect functional estrogen receptor expression in both Western blot and immunohistochemistry. ERPC based techniques are non-radioactive, sensitive, relatively inexpensive, and can be used with all species. The use of both ERPC and ERα- or ERβ-specific antibodies provides complementary information in characterizing estrogen receptors' expression and functional binding to ligand.

KW - BSA

KW - Biotin

KW - Estradiol

KW - Estrogen receptor

KW - Immunohistochemistry

KW - Western blot

UR - https://www.scopus.com/pages/publications/23944482369

U2 - 10.1080/15321810500220894

DO - 10.1080/15321810500220894

M3 - Article

C2 - 16153014

SN - 1532-1819

VL - 26

SP - 295

EP - 301

JO - Journal of Immunoassay and Immunochemistry

JF - Journal of Immunoassay and Immunochemistry

IS - 4

ER -

Method to detect functional estrogen receptor expression using estrogen receptor probing compound

Abstract

Keywords

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