TY - GEN
T1 - Michaelis-menten cytometry for the evaluation of chronic myelogenous Leukemia (CML) at single-cell resolution
AU - Yu, Jinzhu
AU - Antal, Botond
AU - Oh, Ki
AU - Moorthi, Sitapriya
AU - Li, Ling
AU - Luberto, Chiara
AU - Strey, Helmut
AU - Quan, Phenix Lan
AU - Brouzes, Eric
N1 - Publisher Copyright: © 2019 CBMS-0001.
PY - 2019
Y1 - 2019
N2 - We developed a cytometry array that measures the activity of the BCR-ABL pathway in single-cells by exploiting Michaelis-Menten kinetics. BCR-ABL is a constitutively activated kinase responsible for Chronic Myelogenous Leukemia (CML) [1]. Our goal is to test the role of the highly BCR-ABL expressing clones in the drug resistance of CML. We detect the phosphorylation of CrkL, an effector of BCR-ABL, in single-cells via enzymatic amplification within an array of sealed microwells. Using the platform, we characterized the level of BCR-ABL activation in several cell lines using real-time data and advanced data analysis.
AB - We developed a cytometry array that measures the activity of the BCR-ABL pathway in single-cells by exploiting Michaelis-Menten kinetics. BCR-ABL is a constitutively activated kinase responsible for Chronic Myelogenous Leukemia (CML) [1]. Our goal is to test the role of the highly BCR-ABL expressing clones in the drug resistance of CML. We detect the phosphorylation of CrkL, an effector of BCR-ABL, in single-cells via enzymatic amplification within an array of sealed microwells. Using the platform, we characterized the level of BCR-ABL activation in several cell lines using real-time data and advanced data analysis.
KW - Array of microwells
KW - Enzymatic amplification
KW - Michaelis-Menten kinetics
KW - Single-cell analysis
UR - https://www.scopus.com/pages/publications/85094966924
M3 - Conference contribution
T3 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
SP - 482
EP - 483
BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PB - Chemical and Biological Microsystems Society
T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Y2 - 27 October 2019 through 31 October 2019
ER -