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Model phospholipid liposomes to study the β-amyloid-peptide-induced membrane disruption

  • State University of New York Binghamton University

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

8 Scopus citations

Abstract

Model phospholipid liposomes have been utilized widely to study the molecular interactions between peptides and membrane bilayers. In the mechanistic study of Alzheimer’s disease (AD), disruption of neuronal cell membranes has been considered as a major contribution for the β-amyloid (Aβ) peptides’ neurotoxicity. However, clear interpretation of the Aβ-induced cellular membrane at high-resolution level is challenging because of the co-existence of multiple pathways. Here we present the generation of simplified model liposome systems that will facilitate the in-depth mechanistic studies. Protocols for the preparation of model liposomes and the characterization of individual membrane disruption effects will be described.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages355-367
Number of pages13
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1777

Keywords

  • Electrophysiology
  • Fibrillation
  • Ion channels
  • Membrane disruption
  • Phospholipid liposomes
  • lipid mixing
  • β-Amyloid peptides

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