Morphine coupling to invertebrate immunocyte nitric oxide release is dependent on intracellular calcium transients

Fernando E. Nieto-Fernandez; Dwight Mattocks; Francesco Cavani; Michel Salzet; George B. Stefano

doi:10.1016/S0305-0491(99)00074-7

Morphine coupling to invertebrate immunocyte nitric oxide release is dependent on intracellular calcium transients

Fernando E. Nieto-Fernandez
, Dwight Mattocks
, Francesco Cavani
, Michel Salzet
, George B. Stefano

Biological Sciences

Old Westbury

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Morphine significantly stimulated invertebrate immunocyte intracellular calcium level increases in a concentration-dependent manner in cells preloaded with Fura(TM) 2/AM. Morphine's action was blocked by prior exposure of the cells to the opiate receptor antagonist naloxone. Various opioid peptides did not exhibit this ability, indicating a morphine-μ₃ mediated process. In comparing the sequence of events concerning morphine's action in stimulating both [Ca²⁺](i) and NO production in these cells, we found that the first event precedes the second by 42±7 s. The opiate stimulation of [Ca²⁺](i) was attenuated in cells leached of calcium, strongly suggesting that intracellular calcium levels regulate cNOS activity in invertebrate immunocytes. Copyright (C) 1999 Elsevier Science Inc.

Original language	English
Pages (from-to)	295-299
Number of pages	5
Journal	Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume	123
Issue number	3
DOIs	https://doi.org/10.1016/S0305-0491(99)00074-7
State	Published - 1999

Keywords

Calcium transients
Immunocytes
Invertebrates
Morphine
Naloxone
Nitric oxide
Opiate
Opioid
cNOS
μ-Receptor

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10.1016/S0305-0491(99)00074-7

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title = "Morphine coupling to invertebrate immunocyte nitric oxide release is dependent on intracellular calcium transients",

abstract = "Morphine significantly stimulated invertebrate immunocyte intracellular calcium level increases in a concentration-dependent manner in cells preloaded with Fura(TM) 2/AM. Morphine's action was blocked by prior exposure of the cells to the opiate receptor antagonist naloxone. Various opioid peptides did not exhibit this ability, indicating a morphine-μ3 mediated process. In comparing the sequence of events concerning morphine's action in stimulating both [Ca2+](i) and NO production in these cells, we found that the first event precedes the second by 42±7 s. The opiate stimulation of [Ca2+](i) was attenuated in cells leached of calcium, strongly suggesting that intracellular calcium levels regulate cNOS activity in invertebrate immunocytes. Copyright (C) 1999 Elsevier Science Inc.",

keywords = "Calcium transients, Immunocytes, Invertebrates, Morphine, Naloxone, Nitric oxide, Opiate, Opioid, cNOS, μ-Receptor",

author = "Nieto-Fernandez, \{Fernando E.\} and Dwight Mattocks and Francesco Cavani and Michel Salzet and Stefano, \{George B.\}",

year = "1999",

doi = "10.1016/S0305-0491(99)00074-7",

language = "English",

volume = "123",

pages = "295--299",

journal = "Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology",

issn = "0305-0491",

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}

Morphine coupling to invertebrate immunocyte nitric oxide release is dependent on intracellular calcium transients. / Nieto-Fernandez, Fernando E.; Mattocks, Dwight; Cavani, Francesco et al.
In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 123, No. 3, 1999, p. 295-299.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Morphine coupling to invertebrate immunocyte nitric oxide release is dependent on intracellular calcium transients

AU - Nieto-Fernandez, Fernando E.

AU - Mattocks, Dwight

AU - Cavani, Francesco

AU - Salzet, Michel

AU - Stefano, George B.

PY - 1999

Y1 - 1999

N2 - Morphine significantly stimulated invertebrate immunocyte intracellular calcium level increases in a concentration-dependent manner in cells preloaded with Fura(TM) 2/AM. Morphine's action was blocked by prior exposure of the cells to the opiate receptor antagonist naloxone. Various opioid peptides did not exhibit this ability, indicating a morphine-μ3 mediated process. In comparing the sequence of events concerning morphine's action in stimulating both [Ca2+](i) and NO production in these cells, we found that the first event precedes the second by 42±7 s. The opiate stimulation of [Ca2+](i) was attenuated in cells leached of calcium, strongly suggesting that intracellular calcium levels regulate cNOS activity in invertebrate immunocytes. Copyright (C) 1999 Elsevier Science Inc.

AB - Morphine significantly stimulated invertebrate immunocyte intracellular calcium level increases in a concentration-dependent manner in cells preloaded with Fura(TM) 2/AM. Morphine's action was blocked by prior exposure of the cells to the opiate receptor antagonist naloxone. Various opioid peptides did not exhibit this ability, indicating a morphine-μ3 mediated process. In comparing the sequence of events concerning morphine's action in stimulating both [Ca2+](i) and NO production in these cells, we found that the first event precedes the second by 42±7 s. The opiate stimulation of [Ca2+](i) was attenuated in cells leached of calcium, strongly suggesting that intracellular calcium levels regulate cNOS activity in invertebrate immunocytes. Copyright (C) 1999 Elsevier Science Inc.

KW - Calcium transients

KW - Immunocytes

KW - Invertebrates

KW - Morphine

KW - Naloxone

KW - Nitric oxide

KW - Opiate

KW - Opioid

KW - cNOS

KW - μ-Receptor

UR - https://www.scopus.com/pages/publications/0033055419

U2 - 10.1016/S0305-0491(99)00074-7

DO - 10.1016/S0305-0491(99)00074-7

M3 - Article

C2 - 10481258

SN - 0305-0491

VL - 123

SP - 295

EP - 299

JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

IS - 3

ER -

Morphine coupling to invertebrate immunocyte nitric oxide release is dependent on intracellular calcium transients

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