Multifrequency Phase Fluorescence Study of Hapten-Antibody Complexation

Frank V. Bright

doi:10.1021/ac00179a006

Multifrequency Phase Fluorescence Study of Hapten-Antibody Complexation

Frank V. Bright

Chemistry

University at Buffalo

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The rotational dynamics of a phenytoin fluoroimmunoassay system is investigated by using multifrequency phase and modulation fluorescence spectroscopy. Results indicate that the fluorescein label on this relatively small hapten undergoes significant (>45%) local motion even when bound to the phenytoin antibody. We attribute this local motion to unhindered rotation of the fluorescein moiety around the bond attaching it to phenytoin. This local motion affects the sensitivity of a steady-state polarization Immunoassay dramatically.

Original language	English
Pages (from-to)	309-313
Number of pages	5
Journal	Analytical Chemistry
Volume	61
Issue number	4
DOIs	https://doi.org/10.1021/ac00179a006
State	Published - 1989

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@article{11fff406ba724100bab507e2d6e7892e,

title = "Multifrequency Phase Fluorescence Study of Hapten-Antibody Complexation",

abstract = "The rotational dynamics of a phenytoin fluoroimmunoassay system is investigated by using multifrequency phase and modulation fluorescence spectroscopy. Results indicate that the fluorescein label on this relatively small hapten undergoes significant (>45\%) local motion even when bound to the phenytoin antibody. We attribute this local motion to unhindered rotation of the fluorescein moiety around the bond attaching it to phenytoin. This local motion affects the sensitivity of a steady-state polarization Immunoassay dramatically.",

author = "Bright, \{Frank V.\}",

year = "1989",

doi = "10.1021/ac00179a006",

language = "English",

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pages = "309--313",

journal = "Analytical Chemistry",

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publisher = "American Chemical Society",

number = "4",

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T1 - Multifrequency Phase Fluorescence Study of Hapten-Antibody Complexation

AU - Bright, Frank V.

PY - 1989

Y1 - 1989

N2 - The rotational dynamics of a phenytoin fluoroimmunoassay system is investigated by using multifrequency phase and modulation fluorescence spectroscopy. Results indicate that the fluorescein label on this relatively small hapten undergoes significant (>45%) local motion even when bound to the phenytoin antibody. We attribute this local motion to unhindered rotation of the fluorescein moiety around the bond attaching it to phenytoin. This local motion affects the sensitivity of a steady-state polarization Immunoassay dramatically.

AB - The rotational dynamics of a phenytoin fluoroimmunoassay system is investigated by using multifrequency phase and modulation fluorescence spectroscopy. Results indicate that the fluorescein label on this relatively small hapten undergoes significant (>45%) local motion even when bound to the phenytoin antibody. We attribute this local motion to unhindered rotation of the fluorescein moiety around the bond attaching it to phenytoin. This local motion affects the sensitivity of a steady-state polarization Immunoassay dramatically.

UR - https://www.scopus.com/pages/publications/0024505799

U2 - 10.1021/ac00179a006

DO - 10.1021/ac00179a006

M3 - Article

C2 - 2712301

SN - 0003-2700

VL - 61

SP - 309

EP - 313

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 4

ER -

Multifrequency Phase Fluorescence Study of Hapten-Antibody Complexation

Abstract

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