@inproceedings{d3025989a2e6489ea6552490b8e82a79,
title = "Multiplexed fluorescence detection for point of care",
abstract = "We report on the development of a portable fluorescence detection system. By combining a CMOS sensor and crosspolarization scheme, we achieved multiplexed detection with a single white emission LED excitation. We demonstrated fluorescence detection of Fluorescein and Rhodamine B in PDMS channels and achieved 1μM limit of detection (LOD). Microparticles with green and red fluorescence were detected simultaneously without changing the light sources or filters. We were able to clearly resolve microparticles, even if aggregated. The compact microfluorescence approach offers high spatial and spectral resolution, and is suitable for multiplexed detection in point-of-care applications.",
keywords = "CMOS image sensor, cross-polarization, fluorescence detection, particle imaging",
author = "Li Shen and Mike Ratterman and Tyler Stites and David Klotzkin and Ian Papautsky",
year = "2012",
doi = "10.1117/12.910061",
language = "English",
isbn = "9780819488947",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
booktitle = "Microfluidics, BioMEMS, and Medical Microsystems X",
note = "Microfluidics, BioMEMS, and Medical Microsystems X ; Conference date: 23-01-2012 Through 24-01-2012",
}