@inbook{282b2dfa093d48f6b3d3e7ce93d200c3,
title = "Nuclear trafficking of stat proteins visualized by live cell imaging",
abstract = "The ability to observe the dynamic localization of a protein in living cells can provide critical insight to its mode of action and functional molecular interactions. To this purpose, green fluorescent protein (GFP) has served as a powerful tool to tag STAT proteins for microscopic visualization. Live cell imaging with STAT-GFP proteins has contributed to our understanding of signal transduction and the complexities of nuclear transport of STAT proteins. In this report we summarize recent approaches that use GFP-based techniques with live cell imaging to study the mechanisms of STAT nuclear import and export: photoactivation, fluorescence recovery after photobleaching (FRAP), and fluorescence loss in photobleaching (FLIP).",
keywords = "Confocal microscopy, Fluorescence loss in photobleaching, Fluorescence recovery after photobleaching, Green fluorescence protein, Live cell imaging, Photoactivation, Region of interest, Signal transducer and activator of transcription",
author = "Velasco Cimica and Reich, \{Nancy C.\}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2013.",
year = "2013",
doi = "10.1007/978-1-62703-242-1\_14",
language = "English",
isbn = "9781627032414",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "189--202",
booktitle = "JAK-STAT Signalling",
}