OMP decarboxylase-An enigma persists

Brian P. Callahan; Brian G. Miller

doi:10.1016/j.bioorg.2007.07.004

OMP decarboxylase-An enigma persists

Brian P. Callahan
, Brian G. Miller

Chemistry

Binghamton University

Florida State University

Research output: Contribution to journal › Review article › peer-review

26 Scopus citations

Abstract

In 1995, Radzicka and Wolfenden reported that the rate enhancement produced by orotidine 5′-phosphate decarboxylase (ODCase) approaches 10¹⁷, making this enzyme the most effective pure protein catalyst known in Nature [A. Radzicka, R. Wolfenden, Science 267 (1995) 90-93]. Over the last 12 years, there have been many hypotheses put forward to explain that impressive effect. In this perspective, we provide a summary of the reaction pathways under consideration for ODCase, highlight the supporting and refuting data, and suggest experiments designed to further test each of the candidate pathways.

Original language	English
Pages (from-to)	465-469
Number of pages	5
Journal	Bioorganic Chemistry
Volume	35
Issue number	6
DOIs	https://doi.org/10.1016/j.bioorg.2007.07.004
State	Published - Dec 2007

Keywords

Carbanion
Carbene
Decarboxylase
Decarboxylation
Electrostatic stress
Mechanism
OMP
Orotidine
Transition state
Ylide

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10.1016/j.bioorg.2007.07.004

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@article{623ce82482bd4f93bafa45c965747708,

title = "OMP decarboxylase-An enigma persists",

abstract = "In 1995, Radzicka and Wolfenden reported that the rate enhancement produced by orotidine 5′-phosphate decarboxylase (ODCase) approaches 1017, making this enzyme the most effective pure protein catalyst known in Nature [A. Radzicka, R. Wolfenden, Science 267 (1995) 90-93]. Over the last 12 years, there have been many hypotheses put forward to explain that impressive effect. In this perspective, we provide a summary of the reaction pathways under consideration for ODCase, highlight the supporting and refuting data, and suggest experiments designed to further test each of the candidate pathways.",

keywords = "Carbanion, Carbene, Decarboxylase, Decarboxylation, Electrostatic stress, Mechanism, OMP, Orotidine, Transition state, Ylide",

author = "Callahan, \{Brian P.\} and Miller, \{Brian G.\}",

year = "2007",

month = dec,

doi = "10.1016/j.bioorg.2007.07.004",

language = "English",

volume = "35",

pages = "465--469",

journal = "Bioorganic Chemistry",

issn = "0045-2068",

publisher = "Academic Press Inc.",

number = "6",

}

TY - JOUR

T1 - OMP decarboxylase-An enigma persists

AU - Callahan, Brian P.

AU - Miller, Brian G.

PY - 2007/12

Y1 - 2007/12

N2 - In 1995, Radzicka and Wolfenden reported that the rate enhancement produced by orotidine 5′-phosphate decarboxylase (ODCase) approaches 1017, making this enzyme the most effective pure protein catalyst known in Nature [A. Radzicka, R. Wolfenden, Science 267 (1995) 90-93]. Over the last 12 years, there have been many hypotheses put forward to explain that impressive effect. In this perspective, we provide a summary of the reaction pathways under consideration for ODCase, highlight the supporting and refuting data, and suggest experiments designed to further test each of the candidate pathways.

AB - In 1995, Radzicka and Wolfenden reported that the rate enhancement produced by orotidine 5′-phosphate decarboxylase (ODCase) approaches 1017, making this enzyme the most effective pure protein catalyst known in Nature [A. Radzicka, R. Wolfenden, Science 267 (1995) 90-93]. Over the last 12 years, there have been many hypotheses put forward to explain that impressive effect. In this perspective, we provide a summary of the reaction pathways under consideration for ODCase, highlight the supporting and refuting data, and suggest experiments designed to further test each of the candidate pathways.

KW - Carbanion

KW - Carbene

KW - Decarboxylase

KW - Decarboxylation

KW - Electrostatic stress

KW - Mechanism

KW - OMP

KW - Orotidine

KW - Transition state

KW - Ylide

UR - https://www.scopus.com/pages/publications/35448960396

U2 - 10.1016/j.bioorg.2007.07.004

DO - 10.1016/j.bioorg.2007.07.004

M3 - Review article

C2 - 17889251

SN - 0045-2068

VL - 35

SP - 465

EP - 469

JO - Bioorganic Chemistry

JF - Bioorganic Chemistry

IS - 6

ER -

OMP decarboxylase-An enigma persists

Abstract

Keywords

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