On the role of superoxide radical in the mechanism of action of galactose oxidase

Laura D. Kwiatkowski; Daniel J. Kosman

doi:10.1016/0006-291X(73)90152-6

On the role of superoxide radical in the mechanism of action of galactose oxidase

Laura D. Kwiatkowski
, Daniel J. Kosman

Biochemistry

University at Buffalo

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

The inhibition of galactose oxidase by superoxide dismutase is a function of the method of assay, nature of substrate, and composition of incubation and assay mixtures, as well as the concentration of dismutase. A reasonable level of inhibition is attained only when superoxide dismutase is present prior to the onset of catalysis although this effect is not observed under all conditions tried. Peroxidase activates galactose oxidase and blocks its interaction with either superoxide dismutase or catalase. These results further obscure the possible role of superoxide radical in the galactose oxidase reaction.

Original language	English
Pages (from-to)	715-721
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	53
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(73)90152-6
State	Published - Aug 6 1973

Access to Document

10.1016/0006-291X(73)90152-6

Cite this

@article{cb5025e7652b450cacef636ac7bf79ea,

title = "On the role of superoxide radical in the mechanism of action of galactose oxidase",

abstract = "The inhibition of galactose oxidase by superoxide dismutase is a function of the method of assay, nature of substrate, and composition of incubation and assay mixtures, as well as the concentration of dismutase. A reasonable level of inhibition is attained only when superoxide dismutase is present prior to the onset of catalysis although this effect is not observed under all conditions tried. Peroxidase activates galactose oxidase and blocks its interaction with either superoxide dismutase or catalase. These results further obscure the possible role of superoxide radical in the galactose oxidase reaction.",

author = "Kwiatkowski, \{Laura D.\} and Kosman, \{Daniel J.\}",

year = "1973",

month = aug,

day = "6",

doi = "10.1016/0006-291X(73)90152-6",

language = "English",

volume = "53",

pages = "715--721",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - On the role of superoxide radical in the mechanism of action of galactose oxidase

AU - Kwiatkowski, Laura D.

AU - Kosman, Daniel J.

PY - 1973/8/6

Y1 - 1973/8/6

N2 - The inhibition of galactose oxidase by superoxide dismutase is a function of the method of assay, nature of substrate, and composition of incubation and assay mixtures, as well as the concentration of dismutase. A reasonable level of inhibition is attained only when superoxide dismutase is present prior to the onset of catalysis although this effect is not observed under all conditions tried. Peroxidase activates galactose oxidase and blocks its interaction with either superoxide dismutase or catalase. These results further obscure the possible role of superoxide radical in the galactose oxidase reaction.

AB - The inhibition of galactose oxidase by superoxide dismutase is a function of the method of assay, nature of substrate, and composition of incubation and assay mixtures, as well as the concentration of dismutase. A reasonable level of inhibition is attained only when superoxide dismutase is present prior to the onset of catalysis although this effect is not observed under all conditions tried. Peroxidase activates galactose oxidase and blocks its interaction with either superoxide dismutase or catalase. These results further obscure the possible role of superoxide radical in the galactose oxidase reaction.

UR - https://www.scopus.com/pages/publications/0015796836

U2 - 10.1016/0006-291X(73)90152-6

DO - 10.1016/0006-291X(73)90152-6

M3 - Article

C2 - 4738713

SN - 0006-291X

VL - 53

SP - 715

EP - 721

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

On the role of superoxide radical in the mechanism of action of galactose oxidase

Abstract

Access to Document

Other files and links

Fingerprint

Cite this