On the role of the C-terminus of α-calcitonin-gene-related peptide (αCGRP): The structure of des-phenylalaninamide37-αCGRP and its interaction with the CGRP receptor

J. P. O'Connell; S. M. Kelly; D. P. Raleigh; J. A.M. Hubbard; N. C. Price; C. M. Dobson; B. J. Smith

doi:10.1042/bj2910205

On the role of the C-terminus of α-calcitonin-gene-related peptide (αCGRP): The structure of des-phenylalaninamide³⁷-αCGRP and its interaction with the CGRP receptor

J. P. O'Connell
, S. M. Kelly
, D. P. Raleigh
, J. A.M. Hubbard
, N. C. Price
, C. M. Dobson
, B. J. Smith

Chemistry

Stony Brook University

UCB S.A.

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

α-Calcitonin-gene-related peptide (αCGRP) lacking its C-terminal phenylalaninamide residue was found not to bind to its receptor as did full-length (amidated) αCGRP. Investigation of the structure of these peptides by c.d. and n.m.r. revealed no significant difference, so it seemed that the effect of deleting the C-terminal phenylalaninamide on the biological activity of αCGRP was not by disruption of the peptide's structure. Thus the C-terminal phenylalaninamide is an important factor in this ligand-receptor interaction, and the group itself may interact directly with the receptor.

Original language	English
Pages (from-to)	205-210
Number of pages	6
Journal	Biochemical Journal
Volume	291
Issue number	1
DOIs	https://doi.org/10.1042/bj2910205
State	Published - 1993

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title = "On the role of the C-terminus of α-calcitonin-gene-related peptide (αCGRP): The structure of des-phenylalaninamide37-αCGRP and its interaction with the CGRP receptor",

abstract = "α-Calcitonin-gene-related peptide (αCGRP) lacking its C-terminal phenylalaninamide residue was found not to bind to its receptor as did full-length (amidated) αCGRP. Investigation of the structure of these peptides by c.d. and n.m.r. revealed no significant difference, so it seemed that the effect of deleting the C-terminal phenylalaninamide on the biological activity of αCGRP was not by disruption of the peptide's structure. Thus the C-terminal phenylalaninamide is an important factor in this ligand-receptor interaction, and the group itself may interact directly with the receptor.",

author = "O'Connell, \{J. P.\} and Kelly, \{S. M.\} and Raleigh, \{D. P.\} and Hubbard, \{J. A.M.\} and Price, \{N. C.\} and Dobson, \{C. M.\} and Smith, \{B. J.\}",

year = "1993",

doi = "10.1042/bj2910205",

language = "English",

volume = "291",

pages = "205--210",

journal = "Biochemical Journal",

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TY - JOUR

T1 - On the role of the C-terminus of α-calcitonin-gene-related peptide (αCGRP)

T2 - The structure of des-phenylalaninamide37-αCGRP and its interaction with the CGRP receptor

AU - O'Connell, J. P.

AU - Kelly, S. M.

AU - Raleigh, D. P.

AU - Hubbard, J. A.M.

AU - Price, N. C.

AU - Dobson, C. M.

AU - Smith, B. J.

PY - 1993

Y1 - 1993

N2 - α-Calcitonin-gene-related peptide (αCGRP) lacking its C-terminal phenylalaninamide residue was found not to bind to its receptor as did full-length (amidated) αCGRP. Investigation of the structure of these peptides by c.d. and n.m.r. revealed no significant difference, so it seemed that the effect of deleting the C-terminal phenylalaninamide on the biological activity of αCGRP was not by disruption of the peptide's structure. Thus the C-terminal phenylalaninamide is an important factor in this ligand-receptor interaction, and the group itself may interact directly with the receptor.

AB - α-Calcitonin-gene-related peptide (αCGRP) lacking its C-terminal phenylalaninamide residue was found not to bind to its receptor as did full-length (amidated) αCGRP. Investigation of the structure of these peptides by c.d. and n.m.r. revealed no significant difference, so it seemed that the effect of deleting the C-terminal phenylalaninamide on the biological activity of αCGRP was not by disruption of the peptide's structure. Thus the C-terminal phenylalaninamide is an important factor in this ligand-receptor interaction, and the group itself may interact directly with the receptor.

UR - https://www.scopus.com/pages/publications/0027408787

U2 - 10.1042/bj2910205

DO - 10.1042/bj2910205

M3 - Article

C2 - 8385932

SN - 0264-6021

VL - 291

SP - 205

EP - 210

JO - Biochemical Journal

JF - Biochemical Journal

IS - 1

ER -

On the role of the C-terminus of α-calcitonin-gene-related peptide (αCGRP): The structure of des-phenylalaninamide³⁷-αCGRP and its interaction with the CGRP receptor

Abstract

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