Papillomavirus E2 protein interacts with and stimulates human topoisomerase I

Randolph V. Clower; Yan Hu; Thomas Melendy

doi:10.1016/j.virol.2006.02.018

Papillomavirus E2 protein interacts with and stimulates human topoisomerase I

Randolph V. Clower
, Yan Hu
, Thomas Melendy

Microbiology and Immunology

University at Buffalo

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The papillomavirus (PV) E2 protein plays a role in recruiting viral and cellular DNA replication factors, such as PV E1 or RPA to PV genomes. Using both purified proteins and through co-precipitation, it was determined that HPV-11 E2 binds human topoisomerase I. E2 can stimulate topoisomerase I DNA relaxation activity 3- to 4-fold. Conversely, topoisomerase I is unable to stimulate E2 DNA binding. These findings suggest that stimulation of topoisomerase I by E2 may help promote efficient relaxation of the torsional stress induced by PV DNA replication.

Original language	English
Pages (from-to)	13-18
Number of pages	6
Journal	Virology
Volume	348
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2006.02.018
State	Published - Apr 25 2006

Keywords

DNA relaxation
E2
Papillomavirus
Topoisomerase I
Viral DNA replication

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10.1016/j.virol.2006.02.018

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title = "Papillomavirus E2 protein interacts with and stimulates human topoisomerase I",

abstract = "The papillomavirus (PV) E2 protein plays a role in recruiting viral and cellular DNA replication factors, such as PV E1 or RPA to PV genomes. Using both purified proteins and through co-precipitation, it was determined that HPV-11 E2 binds human topoisomerase I. E2 can stimulate topoisomerase I DNA relaxation activity 3- to 4-fold. Conversely, topoisomerase I is unable to stimulate E2 DNA binding. These findings suggest that stimulation of topoisomerase I by E2 may help promote efficient relaxation of the torsional stress induced by PV DNA replication.",

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AU - Clower, Randolph V.

AU - Hu, Yan

AU - Melendy, Thomas

PY - 2006/4/25

Y1 - 2006/4/25

N2 - The papillomavirus (PV) E2 protein plays a role in recruiting viral and cellular DNA replication factors, such as PV E1 or RPA to PV genomes. Using both purified proteins and through co-precipitation, it was determined that HPV-11 E2 binds human topoisomerase I. E2 can stimulate topoisomerase I DNA relaxation activity 3- to 4-fold. Conversely, topoisomerase I is unable to stimulate E2 DNA binding. These findings suggest that stimulation of topoisomerase I by E2 may help promote efficient relaxation of the torsional stress induced by PV DNA replication.

AB - The papillomavirus (PV) E2 protein plays a role in recruiting viral and cellular DNA replication factors, such as PV E1 or RPA to PV genomes. Using both purified proteins and through co-precipitation, it was determined that HPV-11 E2 binds human topoisomerase I. E2 can stimulate topoisomerase I DNA relaxation activity 3- to 4-fold. Conversely, topoisomerase I is unable to stimulate E2 DNA binding. These findings suggest that stimulation of topoisomerase I by E2 may help promote efficient relaxation of the torsional stress induced by PV DNA replication.

KW - DNA relaxation

KW - E2

KW - Papillomavirus

KW - Topoisomerase I

KW - Viral DNA replication

UR - https://www.scopus.com/pages/publications/33646039334

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DO - 10.1016/j.virol.2006.02.018

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VL - 348

SP - 13

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JO - Virology

JF - Virology

IS - 1

ER -

Papillomavirus E2 protein interacts with and stimulates human topoisomerase I

Abstract

Keywords

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