Phylogenetically conserved sequences in the promoter of the rabbit sodium-hydrogen exchanger isoform 1 gene(NHE1/SLC9A1)

Madeleine C. Blaurock; Nancy A. Rebouças; Jacqueline L. Kusnezov; Peter Igarashi

doi:10.1016/0167-4781(95)00075-R

Phylogenetically conserved sequences in the promoter of the rabbit sodium-hydrogen exchanger isoform 1 gene(NHE1/SLC9A1)

Madeleine C. Blaurock
, Nancy A. Rebouças
, Jacqueline L. Kusnezov
, Peter Igarashi

Office of the Dean of Medicine

Stony Brook University

Yale University

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

NHE1 (gene symbol SLC9A1) encodes an isoform of the amiloride-sensitive Na⁺-H⁺ exchanger that is present in many cells and the basolateral membrane of renal epithelia. Expression of NHE1 is modulated in response to chronic metabolic acidosis, growth factors, and phorbol esters. To begin examining the molecular basis for this regulation, a rabbit genomic clone that contained 6 kb of 5′ flanking region, the first exon, and a portion of the first intervening sequence of NHE1 was obtained. The principal transcription start site in native rabbit tissues was located 798 by 5' to the initiation codon. The sequence of the proximal 5′ flanking region of rabbit NHE1 was similar to the human sequence and contained a TATA-box, (G + C)-boxes, homopyrimidine direct repeats, and a putative AP-1 site. When ligated to luciferase and transfected into porcine renal epithelial cells (LLC-PK₁), 708 by of proximal 5′ flanking region exhibited orientation-dependent promoter activity.

Original language	English
Pages (from-to)	159-163
Number of pages	5
Journal	BBA - Gene Structure and Expression
Volume	1262
Issue number	2-3
DOIs	https://doi.org/10.1016/0167-4781(95)00075-R
State	Published - Jun 9 1995

Keywords

Carrier protein
LLC-PK cell
Promoter
Sodium-hydrogen antiporter

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10.1016/0167-4781(95)00075-R

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@article{12a26f5129b44eca8d612b36a842f434,

title = "Phylogenetically conserved sequences in the promoter of the rabbit sodium-hydrogen exchanger isoform 1 gene(NHE1/SLC9A1)",

abstract = "NHE1 (gene symbol SLC9A1) encodes an isoform of the amiloride-sensitive Na+-H+ exchanger that is present in many cells and the basolateral membrane of renal epithelia. Expression of NHE1 is modulated in response to chronic metabolic acidosis, growth factors, and phorbol esters. To begin examining the molecular basis for this regulation, a rabbit genomic clone that contained 6 kb of 5′ flanking region, the first exon, and a portion of the first intervening sequence of NHE1 was obtained. The principal transcription start site in native rabbit tissues was located 798 by 5' to the initiation codon. The sequence of the proximal 5′ flanking region of rabbit NHE1 was similar to the human sequence and contained a TATA-box, (G + C)-boxes, homopyrimidine direct repeats, and a putative AP-1 site. When ligated to luciferase and transfected into porcine renal epithelial cells (LLC-PK1), 708 by of proximal 5′ flanking region exhibited orientation-dependent promoter activity.",

keywords = "Carrier protein, LLC-PK cell, Promoter, Sodium-hydrogen antiporter",

author = "Blaurock, \{Madeleine C.\} and Rebou{\c c}as, \{Nancy A.\} and Kusnezov, \{Jacqueline L.\} and Peter Igarashi",

year = "1995",

month = jun,

day = "9",

doi = "10.1016/0167-4781(95)00075-R",

language = "English",

volume = "1262",

pages = "159--163",

journal = "BBA - Gene Structure and Expression",

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TY - JOUR

T1 - Phylogenetically conserved sequences in the promoter of the rabbit sodium-hydrogen exchanger isoform 1 gene(NHE1/SLC9A1)

AU - Blaurock, Madeleine C.

AU - Rebouças, Nancy A.

AU - Kusnezov, Jacqueline L.

AU - Igarashi, Peter

PY - 1995/6/9

Y1 - 1995/6/9

N2 - NHE1 (gene symbol SLC9A1) encodes an isoform of the amiloride-sensitive Na+-H+ exchanger that is present in many cells and the basolateral membrane of renal epithelia. Expression of NHE1 is modulated in response to chronic metabolic acidosis, growth factors, and phorbol esters. To begin examining the molecular basis for this regulation, a rabbit genomic clone that contained 6 kb of 5′ flanking region, the first exon, and a portion of the first intervening sequence of NHE1 was obtained. The principal transcription start site in native rabbit tissues was located 798 by 5' to the initiation codon. The sequence of the proximal 5′ flanking region of rabbit NHE1 was similar to the human sequence and contained a TATA-box, (G + C)-boxes, homopyrimidine direct repeats, and a putative AP-1 site. When ligated to luciferase and transfected into porcine renal epithelial cells (LLC-PK1), 708 by of proximal 5′ flanking region exhibited orientation-dependent promoter activity.

AB - NHE1 (gene symbol SLC9A1) encodes an isoform of the amiloride-sensitive Na+-H+ exchanger that is present in many cells and the basolateral membrane of renal epithelia. Expression of NHE1 is modulated in response to chronic metabolic acidosis, growth factors, and phorbol esters. To begin examining the molecular basis for this regulation, a rabbit genomic clone that contained 6 kb of 5′ flanking region, the first exon, and a portion of the first intervening sequence of NHE1 was obtained. The principal transcription start site in native rabbit tissues was located 798 by 5' to the initiation codon. The sequence of the proximal 5′ flanking region of rabbit NHE1 was similar to the human sequence and contained a TATA-box, (G + C)-boxes, homopyrimidine direct repeats, and a putative AP-1 site. When ligated to luciferase and transfected into porcine renal epithelial cells (LLC-PK1), 708 by of proximal 5′ flanking region exhibited orientation-dependent promoter activity.

KW - Carrier protein

KW - LLC-PK cell

KW - Promoter

KW - Sodium-hydrogen antiporter

UR - https://www.scopus.com/pages/publications/0029001329

U2 - 10.1016/0167-4781(95)00075-R

DO - 10.1016/0167-4781(95)00075-R

M3 - Article

C2 - 7599192

SN - 0167-4781

VL - 1262

SP - 159

EP - 163

JO - BBA - Gene Structure and Expression

JF - BBA - Gene Structure and Expression

IS - 2-3

ER -

Phylogenetically conserved sequences in the promoter of the rabbit sodium-hydrogen exchanger isoform 1 gene(NHE1/SLC9A1)

Abstract

Keywords

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