Preparation of cell extracts for purification of soluble proteins expressed in E. coli

Clara L. Kielkopf; William Bauer; Ina L. Urbatsch

doi:10.1101/PDB.PROT102178

Preparation of cell extracts for purification of soluble proteins expressed in E. coli

Clara L. Kielkopf
, William Bauer
, Ina L. Urbatsch

Pediatrics

University at Buffalo

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Recovery of intracellular proteins requires disruption of the host cell before the target protein is extracted and isolated. For cells enveloped in cell walls (such as Escherichia coli), vigorous methods are often required. This protocol focuses on E. coli lysis by sonication. Also included are methods for lysis by freeze-thaw and enzymatic treatments.

Original language	English
Pages (from-to)	90-97
Number of pages	8
Journal	Cold Spring Harbor Protocols
Volume	2021
Issue number	2
DOIs	https://doi.org/10.1101/PDB.PROT102178
State	Published - Feb 2021

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title = "Preparation of cell extracts for purification of soluble proteins expressed in E. coli",

abstract = "Recovery of intracellular proteins requires disruption of the host cell before the target protein is extracted and isolated. For cells enveloped in cell walls (such as Escherichia coli), vigorous methods are often required. This protocol focuses on E. coli lysis by sonication. Also included are methods for lysis by freeze-thaw and enzymatic treatments.",

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AU - Kielkopf, Clara L.

AU - Bauer, William

AU - Urbatsch, Ina L.

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N2 - Recovery of intracellular proteins requires disruption of the host cell before the target protein is extracted and isolated. For cells enveloped in cell walls (such as Escherichia coli), vigorous methods are often required. This protocol focuses on E. coli lysis by sonication. Also included are methods for lysis by freeze-thaw and enzymatic treatments.

AB - Recovery of intracellular proteins requires disruption of the host cell before the target protein is extracted and isolated. For cells enveloped in cell walls (such as Escherichia coli), vigorous methods are often required. This protocol focuses on E. coli lysis by sonication. Also included are methods for lysis by freeze-thaw and enzymatic treatments.

UR - https://www.scopus.com/pages/publications/85101253280

U2 - 10.1101/PDB.PROT102178

DO - 10.1101/PDB.PROT102178

M3 - Article

C2 - 33526419

SN - 1940-3402

VL - 2021

SP - 90

EP - 97

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

IS - 2

ER -

Preparation of cell extracts for purification of soluble proteins expressed in E. coli

Abstract

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