Preservation of plant cell ultrastructure during immunolocalization of virus particles

Soumitra Ghoshroy; Vitaly Citovsky

doi:10.1016/S0166-0934(98)00084-6

Preservation of plant cell ultrastructure during immunolocalization of virus particles

Soumitra Ghoshroy
, Vitaly Citovsky

Biochemistry and Cell Biology

Stony Brook University

University of Arizona

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Most immunoelectron microscopy techniques used for ultrastructural analyses of virus-infected plant tissues significantly compromise cellular membranous structures as well as overall contrast and resolution of the image. Here, we describe a protocol which avoids these flaws but retains full antigenicity of the sample. A direct comparison of the conventional and the improved electron immunostaining procedures is presented using tobacco and Arabidopsis thaliana plants infected with turnip vein clearing virus. Copyright (C) 1998 Elsevier Science B.V.

Original language	English
Pages (from-to)	223-229
Number of pages	7
Journal	Journal of Virological Methods
Volume	74
Issue number	2
DOIs	https://doi.org/10.1016/S0166-0934(98)00084-6
State	Published - Oct 1998

Keywords

Coat protein
Electron microscopy
Immunostaining
Plant tissue
Tobamoviruses
Turnip vein clearing virus

Access to Document

10.1016/S0166-0934(98)00084-6

Cite this

@article{0926f04d85134f78ba34e67bb585c0a0,

title = "Preservation of plant cell ultrastructure during immunolocalization of virus particles",

abstract = "Most immunoelectron microscopy techniques used for ultrastructural analyses of virus-infected plant tissues significantly compromise cellular membranous structures as well as overall contrast and resolution of the image. Here, we describe a protocol which avoids these flaws but retains full antigenicity of the sample. A direct comparison of the conventional and the improved electron immunostaining procedures is presented using tobacco and Arabidopsis thaliana plants infected with turnip vein clearing virus. Copyright (C) 1998 Elsevier Science B.V.",

keywords = "Coat protein, Electron microscopy, Immunostaining, Plant tissue, Tobamoviruses, Turnip vein clearing virus",

author = "Soumitra Ghoshroy and Vitaly Citovsky",

year = "1998",

month = oct,

doi = "10.1016/S0166-0934(98)00084-6",

language = "English",

volume = "74",

pages = "223--229",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Preservation of plant cell ultrastructure during immunolocalization of virus particles

AU - Ghoshroy, Soumitra

AU - Citovsky, Vitaly

PY - 1998/10

Y1 - 1998/10

N2 - Most immunoelectron microscopy techniques used for ultrastructural analyses of virus-infected plant tissues significantly compromise cellular membranous structures as well as overall contrast and resolution of the image. Here, we describe a protocol which avoids these flaws but retains full antigenicity of the sample. A direct comparison of the conventional and the improved electron immunostaining procedures is presented using tobacco and Arabidopsis thaliana plants infected with turnip vein clearing virus. Copyright (C) 1998 Elsevier Science B.V.

AB - Most immunoelectron microscopy techniques used for ultrastructural analyses of virus-infected plant tissues significantly compromise cellular membranous structures as well as overall contrast and resolution of the image. Here, we describe a protocol which avoids these flaws but retains full antigenicity of the sample. A direct comparison of the conventional and the improved electron immunostaining procedures is presented using tobacco and Arabidopsis thaliana plants infected with turnip vein clearing virus. Copyright (C) 1998 Elsevier Science B.V.

KW - Coat protein

KW - Electron microscopy

KW - Immunostaining

KW - Plant tissue

KW - Tobamoviruses

KW - Turnip vein clearing virus

UR - https://www.scopus.com/pages/publications/0032193070

U2 - 10.1016/S0166-0934(98)00084-6

DO - 10.1016/S0166-0934(98)00084-6

M3 - Article

C2 - 9779623

SN - 0166-0934

VL - 74

SP - 223

EP - 229

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 2

ER -

Preservation of plant cell ultrastructure during immunolocalization of virus particles

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this