Prophenoloxidase as a reporter of gene expression in Drosophila

John R. True; Artyom Kopp; Sean B. Carroll

doi:10.2144/01305st06

Prophenoloxidase as a reporter of gene expression in Drosophila

John R. True
, Artyom Kopp
, Sean B. Carroll

Ecology and Evolution

Stony Brook University

University of Wisconsin-Madison

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We have developed a novel reporter for the analysis of gene expression using a Drosophila prophenoloxidase (proPO) gene. We have expressed proPO in transgenic flies using the GAL4-UAS system and characterized ectopic expression in imaginal discs. proPO staining is fast and robust, requiring only inexpensive reagents, and performs similarly to β-galactosidase (β-gal) staining. Double staining using UAS-proPO and β-gal reporters is straightforward and provides a convenient method for examining the expression of two genes simultaneously.

Original language	English
Pages (from-to)	1004-1009
Number of pages	6
Journal	BioTechniques
Volume	30
Issue number	5
DOIs	https://doi.org/10.2144/01305st06
State	Published - 2001

Access to Document

10.2144/01305st06

Cite this

@article{21493ada29c44fb0a8f9c302b70748ce,

title = "Prophenoloxidase as a reporter of gene expression in Drosophila",

abstract = "We have developed a novel reporter for the analysis of gene expression using a Drosophila prophenoloxidase (proPO) gene. We have expressed proPO in transgenic flies using the GAL4-UAS system and characterized ectopic expression in imaginal discs. proPO staining is fast and robust, requiring only inexpensive reagents, and performs similarly to β-galactosidase (β-gal) staining. Double staining using UAS-proPO and β-gal reporters is straightforward and provides a convenient method for examining the expression of two genes simultaneously.",

author = "True, \{John R.\} and Artyom Kopp and Carroll, \{Sean B.\}",

year = "2001",

doi = "10.2144/01305st06",

language = "English",

volume = "30",

pages = "1004--1009",

journal = "BioTechniques",

issn = "0736-6205",

publisher = "Eaton Publishing Company",

number = "5",

}

TY - JOUR

T1 - Prophenoloxidase as a reporter of gene expression in Drosophila

AU - True, John R.

AU - Kopp, Artyom

AU - Carroll, Sean B.

PY - 2001

Y1 - 2001

N2 - We have developed a novel reporter for the analysis of gene expression using a Drosophila prophenoloxidase (proPO) gene. We have expressed proPO in transgenic flies using the GAL4-UAS system and characterized ectopic expression in imaginal discs. proPO staining is fast and robust, requiring only inexpensive reagents, and performs similarly to β-galactosidase (β-gal) staining. Double staining using UAS-proPO and β-gal reporters is straightforward and provides a convenient method for examining the expression of two genes simultaneously.

AB - We have developed a novel reporter for the analysis of gene expression using a Drosophila prophenoloxidase (proPO) gene. We have expressed proPO in transgenic flies using the GAL4-UAS system and characterized ectopic expression in imaginal discs. proPO staining is fast and robust, requiring only inexpensive reagents, and performs similarly to β-galactosidase (β-gal) staining. Double staining using UAS-proPO and β-gal reporters is straightforward and provides a convenient method for examining the expression of two genes simultaneously.

UR - https://www.scopus.com/pages/publications/0035015097

U2 - 10.2144/01305st06

DO - 10.2144/01305st06

M3 - Article

C2 - 11355335

SN - 0736-6205

VL - 30

SP - 1004

EP - 1009

JO - BioTechniques

JF - BioTechniques

IS - 5

ER -

Prophenoloxidase as a reporter of gene expression in Drosophila

Abstract

Access to Document

Other files and links

Fingerprint

Cite this