Purification and nuclear localization of a type I topoisomerase from Crithidia fasciculata

Thomas Melendy; Dan S. Ray

doi:10.1016/0166-6851(87)90108-3

Purification and nuclear localization of a type I topoisomerase from Crithidia fasciculata

Thomas Melendy
, Dan S. Ray

Microbiology and Immunology

University at Buffalo

University of California at Los Angeles

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

A type I topoisomerase has been purified to near homogeneity from the trypanosomatid Crithidia fasciculata. The topoisomerase consists of a single 79 kDa polypeptide. The enzyme does not require divalent cations but is stimulated 10-20 fold by the presence of MgCl₂. ATP does not affect enzyme activity, while Berenil, N-ethylmaleimide and ethidium bromide are inhibitory. Immunoblots show that the 79 kDa polypeptide is the most prevalent form of the enzyme in extracts of freshly lysed cells and is immunogenically conserved among a variety of trypanosomes. The topoisomerase was localized to the cell nucleus by double antibody immunofluorescence.

Original language	English
Pages (from-to)	215-225
Number of pages	11
Journal	Molecular and Biochemical Parasitology
Volume	24
Issue number	2
DOIs	https://doi.org/10.1016/0166-6851(87)90108-3
State	Published - Jun 1987

Keywords

Crithidia fasciculata
Nucleus
Topoisomerase

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10.1016/0166-6851(87)90108-3

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title = "Purification and nuclear localization of a type I topoisomerase from Crithidia fasciculata",

abstract = "A type I topoisomerase has been purified to near homogeneity from the trypanosomatid Crithidia fasciculata. The topoisomerase consists of a single 79 kDa polypeptide. The enzyme does not require divalent cations but is stimulated 10-20 fold by the presence of MgCl2. ATP does not affect enzyme activity, while Berenil, N-ethylmaleimide and ethidium bromide are inhibitory. Immunoblots show that the 79 kDa polypeptide is the most prevalent form of the enzyme in extracts of freshly lysed cells and is immunogenically conserved among a variety of trypanosomes. The topoisomerase was localized to the cell nucleus by double antibody immunofluorescence.",

keywords = "Crithidia fasciculata, Nucleus, Topoisomerase",

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T1 - Purification and nuclear localization of a type I topoisomerase from Crithidia fasciculata

AU - Melendy, Thomas

AU - Ray, Dan S.

PY - 1987/6

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N2 - A type I topoisomerase has been purified to near homogeneity from the trypanosomatid Crithidia fasciculata. The topoisomerase consists of a single 79 kDa polypeptide. The enzyme does not require divalent cations but is stimulated 10-20 fold by the presence of MgCl2. ATP does not affect enzyme activity, while Berenil, N-ethylmaleimide and ethidium bromide are inhibitory. Immunoblots show that the 79 kDa polypeptide is the most prevalent form of the enzyme in extracts of freshly lysed cells and is immunogenically conserved among a variety of trypanosomes. The topoisomerase was localized to the cell nucleus by double antibody immunofluorescence.

AB - A type I topoisomerase has been purified to near homogeneity from the trypanosomatid Crithidia fasciculata. The topoisomerase consists of a single 79 kDa polypeptide. The enzyme does not require divalent cations but is stimulated 10-20 fold by the presence of MgCl2. ATP does not affect enzyme activity, while Berenil, N-ethylmaleimide and ethidium bromide are inhibitory. Immunoblots show that the 79 kDa polypeptide is the most prevalent form of the enzyme in extracts of freshly lysed cells and is immunogenically conserved among a variety of trypanosomes. The topoisomerase was localized to the cell nucleus by double antibody immunofluorescence.

KW - Crithidia fasciculata

KW - Nucleus

KW - Topoisomerase

UR - https://www.scopus.com/pages/publications/0023356366

U2 - 10.1016/0166-6851(87)90108-3

DO - 10.1016/0166-6851(87)90108-3

M3 - Article

C2 - 3041212

SN - 0166-6851

VL - 24

SP - 215

EP - 225

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

IS - 2

ER -

Purification and nuclear localization of a type I topoisomerase from Crithidia fasciculata

Abstract

Keywords

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