Quantitative changes in interleukin proteins following focal stroke in the rat

Jeffrey J. Legos; Robert G. Whitmore; Joseph A. Erhardt; Andrew A. Parsons; Ronald F. Tuma; Frank C. Barone

doi:10.1016/S0304-3940(00)00907-1

Quantitative changes in interleukin proteins following focal stroke in the rat

Jeffrey J. Legos
, Robert G. Whitmore
, Joseph A. Erhardt
, Andrew A. Parsons
, Ronald F. Tuma
, Frank C. Barone

Neurology

SUNY Downstate Health Sciences University

Temple University
SmithKline Beecham P.

Research output: Contribution to journal › Article › peer-review

86 Scopus citations

Abstract

The aim of the present study was to quantitate the temporal changes in protein concentration for interleukin (IL)-1α, IL-1β, IL-1ra, and IL-6 from 1 h to 15 days following focal ischemia. Protein expression was evaluated by enzyme-linked immunosorbent assay utilizing newly available rat antibodies. There were no detectable basal levels of IL-1α, 1L-1β, or IL-6 in the sham- operated or non-ischemic control cortex. IL-1β (increased significantly (P < 0.05) as early as 4 h and peaked at 3 to 5 days. IL-1α (increased significantly (P < 0.05) at 3 days. IL-6 increased early and peaked at 24 h (P < 0.05). IL-1ra increased significantly (P < 0.05) over basal levels from 12 h to 5 days. The present study provides the first quantitative determination of interleukin protein concentrations in the rat brain following focal stroke and demonstrates that this technology is now available for mechanistic studies in neuroprotection. (C) 2000 Elsevier Science Ireland Ltd.

Original language	English
Pages (from-to)	189-192
Number of pages	4
Journal	Neuroscience Letters
Volume	282
Issue number	3
DOIs	https://doi.org/10.1016/S0304-3940(00)00907-1
State	Published - Mar 24 2000

Keywords

Cytokines
Focal cerebral ischemia
Interleukin-1
Interleukin-1 receptor antagonist
Interleukin-6
Rats

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10.1016/S0304-3940(00)00907-1

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title = "Quantitative changes in interleukin proteins following focal stroke in the rat",

abstract = "The aim of the present study was to quantitate the temporal changes in protein concentration for interleukin (IL)-1α, IL-1β, IL-1ra, and IL-6 from 1 h to 15 days following focal ischemia. Protein expression was evaluated by enzyme-linked immunosorbent assay utilizing newly available rat antibodies. There were no detectable basal levels of IL-1α, 1L-1β, or IL-6 in the sham- operated or non-ischemic control cortex. IL-1β (increased significantly (P < 0.05) as early as 4 h and peaked at 3 to 5 days. IL-1α (increased significantly (P < 0.05) at 3 days. IL-6 increased early and peaked at 24 h (P < 0.05). IL-1ra increased significantly (P < 0.05) over basal levels from 12 h to 5 days. The present study provides the first quantitative determination of interleukin protein concentrations in the rat brain following focal stroke and demonstrates that this technology is now available for mechanistic studies in neuroprotection. (C) 2000 Elsevier Science Ireland Ltd.",

keywords = "Cytokines, Focal cerebral ischemia, Interleukin-1, Interleukin-1 receptor antagonist, Interleukin-6, Rats",

author = "Legos, \{Jeffrey J.\} and Whitmore, \{Robert G.\} and Erhardt, \{Joseph A.\} and Parsons, \{Andrew A.\} and Tuma, \{Ronald F.\} and Barone, \{Frank C.\}",

year = "2000",

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doi = "10.1016/S0304-3940(00)00907-1",

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T1 - Quantitative changes in interleukin proteins following focal stroke in the rat

AU - Legos, Jeffrey J.

AU - Whitmore, Robert G.

AU - Erhardt, Joseph A.

AU - Parsons, Andrew A.

AU - Tuma, Ronald F.

AU - Barone, Frank C.

PY - 2000/3/24

Y1 - 2000/3/24

N2 - The aim of the present study was to quantitate the temporal changes in protein concentration for interleukin (IL)-1α, IL-1β, IL-1ra, and IL-6 from 1 h to 15 days following focal ischemia. Protein expression was evaluated by enzyme-linked immunosorbent assay utilizing newly available rat antibodies. There were no detectable basal levels of IL-1α, 1L-1β, or IL-6 in the sham- operated or non-ischemic control cortex. IL-1β (increased significantly (P < 0.05) as early as 4 h and peaked at 3 to 5 days. IL-1α (increased significantly (P < 0.05) at 3 days. IL-6 increased early and peaked at 24 h (P < 0.05). IL-1ra increased significantly (P < 0.05) over basal levels from 12 h to 5 days. The present study provides the first quantitative determination of interleukin protein concentrations in the rat brain following focal stroke and demonstrates that this technology is now available for mechanistic studies in neuroprotection. (C) 2000 Elsevier Science Ireland Ltd.

AB - The aim of the present study was to quantitate the temporal changes in protein concentration for interleukin (IL)-1α, IL-1β, IL-1ra, and IL-6 from 1 h to 15 days following focal ischemia. Protein expression was evaluated by enzyme-linked immunosorbent assay utilizing newly available rat antibodies. There were no detectable basal levels of IL-1α, 1L-1β, or IL-6 in the sham- operated or non-ischemic control cortex. IL-1β (increased significantly (P < 0.05) as early as 4 h and peaked at 3 to 5 days. IL-1α (increased significantly (P < 0.05) at 3 days. IL-6 increased early and peaked at 24 h (P < 0.05). IL-1ra increased significantly (P < 0.05) over basal levels from 12 h to 5 days. The present study provides the first quantitative determination of interleukin protein concentrations in the rat brain following focal stroke and demonstrates that this technology is now available for mechanistic studies in neuroprotection. (C) 2000 Elsevier Science Ireland Ltd.

KW - Cytokines

KW - Focal cerebral ischemia

KW - Interleukin-1

KW - Interleukin-1 receptor antagonist

KW - Interleukin-6

KW - Rats

UR - https://www.scopus.com/pages/publications/0034708392

U2 - 10.1016/S0304-3940(00)00907-1

DO - 10.1016/S0304-3940(00)00907-1

M3 - Article

C2 - 10717423

SN - 0304-3940

VL - 282

SP - 189

EP - 192

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 3

ER -

Quantitative changes in interleukin proteins following focal stroke in the rat

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Keywords

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