@inproceedings{47612cde2c7f4b1fa82f6e552b8b195a,
title = "Rapid detection of kinase translocation at the single cell level on a microfluidic chip",
abstract = "Detection of localization of kinases within cells has been largely carried out based on imaging of a low number of cells or subcellular fractionation/Western blotting which provide only the average behaviors of cell populations. Here we demonstrate a new tool, referred to as microfluidic electroporative flow cytometry (EFC), to detect the translocation of an EGFP-tagged tyrosine kinase, Syk, to the plasma membrane in B cells at single cell level. Due to the frequent involvement of kinase translocations in disease processes such as oncogenesis, our approach will have utility for kinase-related drug discovery and tumor diagnosis and staging.",
keywords = "Electroporation, Flow cytometry, Kinase, Microfluidics, Syk, Translocation",
author = "Jun Wang and Ning Bao and Paris, \{Leela L.\} and Wang, \{Hsiang Yu\} and Geahlen, \{Robert L.\} and Chang Lu",
note = "Publisher Copyright: {\textcopyright} 2007 CBMS.; 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 ; Conference date: 07-10-2007 Through 11-10-2007",
year = "2007",
language = "English",
series = "Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007",
publisher = "Chemical and Biological Microsystems Society",
pages = "670--672",
editor = "Jean-Louis Viovy and Patrick Tabeling and Stephanie Descroix and Laurent Malaquin",
booktitle = "Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007",
}