Separation of [1-3H]cellooligosaccharides by thin-layer chromatography: Assay for cellulolytic enzymes

William J. Chirico; Ross D. Brown

doi:10.1016/0003-2697(85)90509-3

Separation of [1-³H]cellooligosaccharides by thin-layer chromatography: Assay for cellulolytic enzymes

William J. Chirico
, Ross D. Brown

Cell / Cellular Biology and Anatomical Sciences

SUNY Downstate Health Sciences University

University of Florida

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

A thin-layer chromatographic method for the separation with high resolution of [1-³H]-celloligosaccharides on silica gel plates has been developed. Reducing end-labeled glucose through cellohexaose were separated on silica gel plates with three ascents of ethyl acetate:water:methanol (40:15:20; v:v) and each was extracted with an efficiency of 88 ± 3%. Separations of cellooligosaccharides using other adsorbents, solvents, and impregnants are also described. This thin-layer chromatographic method facilitated analysis of the activity of cellulolytic enzymes.

Original language	English
Pages (from-to)	264-272
Number of pages	9
Journal	Analytical Biochemistry
Volume	150
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(85)90509-3
State	Published - Nov 1 1985

Keywords

carbohydrates
cellooligosaccharides
cellulases
thin-layer chromatography

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10.1016/0003-2697(85)90509-3

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title = "Separation of [1-3H]cellooligosaccharides by thin-layer chromatography: Assay for cellulolytic enzymes",

abstract = "A thin-layer chromatographic method for the separation with high resolution of [1-3H]-celloligosaccharides on silica gel plates has been developed. Reducing end-labeled glucose through cellohexaose were separated on silica gel plates with three ascents of ethyl acetate:water:methanol (40:15:20; v:v) and each was extracted with an efficiency of 88 ± 3\%. Separations of cellooligosaccharides using other adsorbents, solvents, and impregnants are also described. This thin-layer chromatographic method facilitated analysis of the activity of cellulolytic enzymes.",

keywords = "carbohydrates, cellooligosaccharides, cellulases, thin-layer chromatography",

author = "Chirico, \{William J.\} and Brown, \{Ross D.\}",

year = "1985",

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doi = "10.1016/0003-2697(85)90509-3",

language = "English",

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journal = "Analytical Biochemistry",

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T1 - Separation of [1-3H]cellooligosaccharides by thin-layer chromatography

T2 - Assay for cellulolytic enzymes

AU - Chirico, William J.

AU - Brown, Ross D.

PY - 1985/11/1

Y1 - 1985/11/1

N2 - A thin-layer chromatographic method for the separation with high resolution of [1-3H]-celloligosaccharides on silica gel plates has been developed. Reducing end-labeled glucose through cellohexaose were separated on silica gel plates with three ascents of ethyl acetate:water:methanol (40:15:20; v:v) and each was extracted with an efficiency of 88 ± 3%. Separations of cellooligosaccharides using other adsorbents, solvents, and impregnants are also described. This thin-layer chromatographic method facilitated analysis of the activity of cellulolytic enzymes.

AB - A thin-layer chromatographic method for the separation with high resolution of [1-3H]-celloligosaccharides on silica gel plates has been developed. Reducing end-labeled glucose through cellohexaose were separated on silica gel plates with three ascents of ethyl acetate:water:methanol (40:15:20; v:v) and each was extracted with an efficiency of 88 ± 3%. Separations of cellooligosaccharides using other adsorbents, solvents, and impregnants are also described. This thin-layer chromatographic method facilitated analysis of the activity of cellulolytic enzymes.

KW - carbohydrates

KW - cellooligosaccharides

KW - cellulases

KW - thin-layer chromatography

UR - https://www.scopus.com/pages/publications/0022273135

U2 - 10.1016/0003-2697(85)90509-3

DO - 10.1016/0003-2697(85)90509-3

M3 - Article

C2 - 3004251

SN - 0003-2697

VL - 150

SP - 264

EP - 272

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Separation of [1-³H]cellooligosaccharides by thin-layer chromatography: Assay for cellulolytic enzymes

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Keywords

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