Site-specific fluorescence labelling of RNA using bio-orthogonal reaction of trans -cyclooctene and tetrazine

P. A. Asare-Okai; E. Agustin; D. Fabris; M. Royzen

doi:10.1039/c4cc02435d

Site-specific fluorescence labelling of RNA using bio-orthogonal reaction of trans -cyclooctene and tetrazine

P. A. Asare-Okai
, E. Agustin
, D. Fabris
, M. Royzen

Chemistry

University at Albany

SUNY Albany

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

This communication describes a general approach for site-specific fluorescence labelling of RNA using a cytidine triphosphate (CTP) analogue derivatized with a trans -cyclooctene group. The analogue was efficiently incorporated into a model RNA strand using in vitro transcription. Bio-orthogonal reaction with fluorescein-labelled tetrazine was utilized to fluorescently tag the synthetic RNA strand.

Original language	English
Pages (from-to)	7844-7847
Number of pages	4
Journal	Chemical Communications
Volume	50
Issue number	58
DOIs	https://doi.org/10.1039/c4cc02435d
State	Published - Jun 24 2014

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abstract = "This communication describes a general approach for site-specific fluorescence labelling of RNA using a cytidine triphosphate (CTP) analogue derivatized with a trans -cyclooctene group. The analogue was efficiently incorporated into a model RNA strand using in vitro transcription. Bio-orthogonal reaction with fluorescein-labelled tetrazine was utilized to fluorescently tag the synthetic RNA strand.",

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AU - Asare-Okai, P. A.

AU - Agustin, E.

AU - Fabris, D.

AU - Royzen, M.

PY - 2014/6/24

Y1 - 2014/6/24

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AB - This communication describes a general approach for site-specific fluorescence labelling of RNA using a cytidine triphosphate (CTP) analogue derivatized with a trans -cyclooctene group. The analogue was efficiently incorporated into a model RNA strand using in vitro transcription. Bio-orthogonal reaction with fluorescein-labelled tetrazine was utilized to fluorescently tag the synthetic RNA strand.

UR - https://www.scopus.com/pages/publications/84903169661

U2 - 10.1039/c4cc02435d

DO - 10.1039/c4cc02435d

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JF - Chemical Communications

IS - 58

ER -

Site-specific fluorescence labelling of RNA using bio-orthogonal reaction of trans -cyclooctene and tetrazine

Abstract

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