Small, acid-soluble proteins as biomarkers in mass spectrometry analysis of Bacillus spores

Yetrib Hathout; Barbara Setlow; Rosa Martha Cabrera-Martinez; Catherine Fenselau; Peter Setlow

doi:10.1128/AEM.69.2.1100-1107.2003

Small, acid-soluble proteins as biomarkers in mass spectrometry analysis of Bacillus spores

Yetrib Hathout
, Barbara Setlow
, Rosa Martha Cabrera-Martinez
, Catherine Fenselau
, Peter Setlow

Pharmaceutical Sciences

Binghamton University

University of Connecticut
Instituto Politécnico Nacional
University of Maryland, College Park

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

The use of 1 N HCl for extraction of small, acid-soluble proteins (SASP) from different Bacillus spore species was examined. The extracts were analyzed by high-performance liquid chromatography and matrix-assisted laser desorption mass spectrometry and were found to be both qualitatively and quantitatively superior to extraction by acetonitrile-5% trifluoroacetic acid (70:30, vol/vol). Both major and minor α/β and γ-type SASP were characterized by their molecular masses or tryptic peptide maps and by searches of both protein and unannotated genome databases. For all but 1 pair (B. cereus T and B. thuringiensis subsp. Kurstaki) among the 11 variants studied the suites of SASP masses are distinctive, consistent with the use of these proteins as potential biomarkers for spore identification by mass spectrometry.

Original language	English
Pages (from-to)	1100-1107
Number of pages	8
Journal	Applied and Environmental Microbiology
Volume	69
Issue number	2
DOIs	https://doi.org/10.1128/AEM.69.2.1100-1107.2003
State	Published - Feb 1 2003

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10.1128/AEM.69.2.1100-1107.2003

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title = "Small, acid-soluble proteins as biomarkers in mass spectrometry analysis of Bacillus spores",

abstract = "The use of 1 N HCl for extraction of small, acid-soluble proteins (SASP) from different Bacillus spore species was examined. The extracts were analyzed by high-performance liquid chromatography and matrix-assisted laser desorption mass spectrometry and were found to be both qualitatively and quantitatively superior to extraction by acetonitrile-5\% trifluoroacetic acid (70:30, vol/vol). Both major and minor α/β and γ-type SASP were characterized by their molecular masses or tryptic peptide maps and by searches of both protein and unannotated genome databases. For all but 1 pair (B. cereus T and B. thuringiensis subsp. Kurstaki) among the 11 variants studied the suites of SASP masses are distinctive, consistent with the use of these proteins as potential biomarkers for spore identification by mass spectrometry.",

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AU - Setlow, Barbara

AU - Cabrera-Martinez, Rosa Martha

AU - Fenselau, Catherine

AU - Setlow, Peter

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Small, acid-soluble proteins as biomarkers in mass spectrometry analysis of Bacillus spores

Abstract

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