Solid-State Analysis of Eumelanin Biopolymers by Electron Spectroscopy for Chemical Analysis

Michael B. Clark; Joseph A. Gardella; Thomas M. Schultz; Dilip G. Patil; Lawrence Salvati

doi:10.1021/ac00208a011

Solid-State Analysis of Eumelanin Biopolymers by Electron Spectroscopy for Chemical Analysis

Michael B. Clark
, Joseph A. Gardella
, Thomas M. Schultz
, Dilip G. Patil
, Lawrence Salvati

Chemistry

University at Buffalo

SUNY Buffalo
Clairol Research Laboratories
PerkinElmer, Inc.
Dow Chemical
Laboratories
Zotos International
University of Delaware
Physical Electronics Laboratories

Research output: Contribution to journal › Article › peer-review

104 Scopus citations

Abstract

The single most misleading aspect of structure elucidation of naturally occurring biopolymers lies in the removal and preparation of the sample for chemical and physical composition analysis. We present a method for the application of electron spectroscopy for chemical analysis (ESCA or XPS (X-ray photoelectron spectroscopy)) to elucidate the chemical composition and organic functional group distribution within one type of blopolymer, eumelanin. Qualitative and quantitative elemental analyses were calibrated with model compounds. In addition, the relative proportions of the different carbon, oxygen, and nitrogen functionalities within these eumelanin precursor model compounds and the polymers generated from them are presented. The ESCA results showed that eumelanin has structural and composltonal components consisting of both aromatic and partially oxidized units of 5,6-dihydroxylndole and 5,6-dihydroxyindole-2-carboxylic acid.

Original language	English
Pages (from-to)	949-956
Number of pages	8
Journal	Analytical Chemistry
Volume	62
Issue number	9
DOIs	https://doi.org/10.1021/ac00208a011
State	Published - Apr 15 1990

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title = "Solid-State Analysis of Eumelanin Biopolymers by Electron Spectroscopy for Chemical Analysis",

abstract = "The single most misleading aspect of structure elucidation of naturally occurring biopolymers lies in the removal and preparation of the sample for chemical and physical composition analysis. We present a method for the application of electron spectroscopy for chemical analysis (ESCA or XPS (X-ray photoelectron spectroscopy)) to elucidate the chemical composition and organic functional group distribution within one type of blopolymer, eumelanin. Qualitative and quantitative elemental analyses were calibrated with model compounds. In addition, the relative proportions of the different carbon, oxygen, and nitrogen functionalities within these eumelanin precursor model compounds and the polymers generated from them are presented. The ESCA results showed that eumelanin has structural and composltonal components consisting of both aromatic and partially oxidized units of 5,6-dihydroxylndole and 5,6-dihydroxyindole-2-carboxylic acid.",

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doi = "10.1021/ac00208a011",

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T1 - Solid-State Analysis of Eumelanin Biopolymers by Electron Spectroscopy for Chemical Analysis

AU - Clark, Michael B.

AU - Gardella, Joseph A.

AU - Schultz, Thomas M.

AU - Patil, Dilip G.

AU - Salvati, Lawrence

PY - 1990/4/15

Y1 - 1990/4/15

N2 - The single most misleading aspect of structure elucidation of naturally occurring biopolymers lies in the removal and preparation of the sample for chemical and physical composition analysis. We present a method for the application of electron spectroscopy for chemical analysis (ESCA or XPS (X-ray photoelectron spectroscopy)) to elucidate the chemical composition and organic functional group distribution within one type of blopolymer, eumelanin. Qualitative and quantitative elemental analyses were calibrated with model compounds. In addition, the relative proportions of the different carbon, oxygen, and nitrogen functionalities within these eumelanin precursor model compounds and the polymers generated from them are presented. The ESCA results showed that eumelanin has structural and composltonal components consisting of both aromatic and partially oxidized units of 5,6-dihydroxylndole and 5,6-dihydroxyindole-2-carboxylic acid.

AB - The single most misleading aspect of structure elucidation of naturally occurring biopolymers lies in the removal and preparation of the sample for chemical and physical composition analysis. We present a method for the application of electron spectroscopy for chemical analysis (ESCA or XPS (X-ray photoelectron spectroscopy)) to elucidate the chemical composition and organic functional group distribution within one type of blopolymer, eumelanin. Qualitative and quantitative elemental analyses were calibrated with model compounds. In addition, the relative proportions of the different carbon, oxygen, and nitrogen functionalities within these eumelanin precursor model compounds and the polymers generated from them are presented. The ESCA results showed that eumelanin has structural and composltonal components consisting of both aromatic and partially oxidized units of 5,6-dihydroxylndole and 5,6-dihydroxyindole-2-carboxylic acid.

UR - https://www.scopus.com/pages/publications/0000432077

U2 - 10.1021/ac00208a011

DO - 10.1021/ac00208a011

M3 - Article

SN - 0003-2700

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SP - 949

EP - 956

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 9

ER -

Solid-State Analysis of Eumelanin Biopolymers by Electron Spectroscopy for Chemical Analysis

Abstract

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