TY - GEN
T1 - Statistical particle tracking velocimetry using molecular and quantum dot tracer particles
AU - Guasto, Jeffrey S.
AU - Huang, Peter
AU - Breuer, Kenneth S.
PY - 2005
Y1 - 2005
N2 - We present the theory and experimental validation of a particle tracking velocimetry algorithm developed for application with nanometer-sized tracer particles such as fluorescent molecules and quantum dots (QDs). Traditional algorithms are challenged by extremely small tracers due to difficulties in determining the particle center, shot noise, high drop-in/drop-out and, in the case of quantum dots, fluorescence intermittency (blinking). The algorithms presented here determine real velocity distributions from measured particle displacement distributions by statistically removing randomly distributed tracking events. The theory was verified through tracking experiments using 54 nm flourescent dextran molecules and 6 nm QDs.
AB - We present the theory and experimental validation of a particle tracking velocimetry algorithm developed for application with nanometer-sized tracer particles such as fluorescent molecules and quantum dots (QDs). Traditional algorithms are challenged by extremely small tracers due to difficulties in determining the particle center, shot noise, high drop-in/drop-out and, in the case of quantum dots, fluorescence intermittency (blinking). The algorithms presented here determine real velocity distributions from measured particle displacement distributions by statistically removing randomly distributed tracking events. The theory was verified through tracking experiments using 54 nm flourescent dextran molecules and 6 nm QDs.
UR - https://www.scopus.com/pages/publications/33645980931
U2 - 10.1115/IMECE2005-80051
DO - 10.1115/IMECE2005-80051
M3 - Conference contribution
SN - 0791842193
SN - 9780791842195
T3 - American Society of Mechanical Engineers, Fluids Engineering Division (Publication) FED
SP - 235
EP - 244
BT - American Society of Mechanical Engineers, Fluids Engineering Division (Publication) FED
T2 - 2005 ASME International Mechanical Engineering Congress and Exposition, IMECE 2005
Y2 - 5 November 2005 through 11 November 2005
ER -