Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp

Marcus Gallagher-Jones; Calina Glynn; David R. Boyer; Michael W. Martynowycz; Evelyn Hernandez; Jennifer Miao; Chih Te Zee; Irina V. Novikova; Lukasz Goldschmidt; Heather T. McFarlane; Gustavo F. Helguera; James E. Evans; Michael R. Sawaya; Duilio Cascio; David S. Eisenberg; Tamir Gonen; Jose A. Rodriguez

doi:10.1038/s41594-017-0018-0

Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp

Marcus Gallagher-Jones
, Calina Glynn
, David R. Boyer
, Michael W. Martynowycz
, Evelyn Hernandez
, Jennifer Miao
, Chih Te Zee
, Irina V. Novikova
, Lukasz Goldschmidt
, Heather T. McFarlane
, Gustavo F. Helguera
, James E. Evans
, Michael R. Sawaya
, Duilio Cascio
, David S. Eisenberg
, Tamir Gonen
, Jose A. Rodriguez

Department of Structural Biology

University at Buffalo

University of California
University of California at Los Angeles
Environmental Molecular Sciences Laboratory
Laboratorio de Biologia Del Comportamiento
Howard Hughes Medical Institute

Research output: Contribution to journal › Article › peer-review

76 Scopus citations

Abstract

The atomic structure of the infectious, protease-resistant, β-sheet-rich and fibrillar mammalian prion remains unknown. Through the cryo-EM method MicroED, we reveal the sub-ångström-resolution structure of a protofibril formed by a wild-type segment from the β2-α2 loop of the bank vole prion protein. The structure of this protofibril reveals a stabilizing network of hydrogen bonds that link polar zippers within a sheet, producing motifs we have named 'polar clasps'.

Original language	English
Pages (from-to)	131-134
Number of pages	4
Journal	Nature Structural and Molecular Biology
Volume	25
Issue number	2
DOIs	https://doi.org/10.1038/s41594-017-0018-0
State	Published - Feb 1 2018

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Gallagher-Jones, M., Glynn, C., Boyer, D. R., Martynowycz, M. W., Hernandez, E., Miao, J., Zee, C. T., Novikova, I. V., Goldschmidt, L., McFarlane, H. T., Helguera, G. F., Evans, J. E., Sawaya, M. R., Cascio, D., Eisenberg, D. S., Gonen, T., & Rodriguez, J. A. (2018). Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp. Nature Structural and Molecular Biology, 25(2), 131-134. https://doi.org/10.1038/s41594-017-0018-0

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title = "Sub-{\aa}ngstr{\"o}m cryo-EM structure of a prion protofibril reveals a polar clasp",

abstract = "The atomic structure of the infectious, protease-resistant, β-sheet-rich and fibrillar mammalian prion remains unknown. Through the cryo-EM method MicroED, we reveal the sub-{\aa}ngstr{\"o}m-resolution structure of a protofibril formed by a wild-type segment from the β2-α2 loop of the bank vole prion protein. The structure of this protofibril reveals a stabilizing network of hydrogen bonds that link polar zippers within a sheet, producing motifs we have named 'polar clasps'.",

author = "Marcus Gallagher-Jones and Calina Glynn and Boyer, \{David R.\} and Martynowycz, \{Michael W.\} and Evelyn Hernandez and Jennifer Miao and Zee, \{Chih Te\} and Novikova, \{Irina V.\} and Lukasz Goldschmidt and McFarlane, \{Heather T.\} and Helguera, \{Gustavo F.\} and Evans, \{James E.\} and Sawaya, \{Michael R.\} and Duilio Cascio and Eisenberg, \{David S.\} and Tamir Gonen and Rodriguez, \{Jose A.\}",

note = "Publisher Copyright: {\textcopyright} 2018 The Author(s).",

year = "2018",

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day = "1",

doi = "10.1038/s41594-017-0018-0",

language = "English",

volume = "25",

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Gallagher-Jones, M, Glynn, C, Boyer, DR, Martynowycz, MW, Hernandez, E, Miao, J, Zee, CT, Novikova, IV, Goldschmidt, L, McFarlane, HT, Helguera, GF, Evans, JE, Sawaya, MR, Cascio, D, Eisenberg, DS, Gonen, T & Rodriguez, JA 2018, 'Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp', Nature Structural and Molecular Biology, vol. 25, no. 2, pp. 131-134. https://doi.org/10.1038/s41594-017-0018-0

TY - JOUR

T1 - Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp

AU - Gallagher-Jones, Marcus

AU - Glynn, Calina

AU - Boyer, David R.

AU - Martynowycz, Michael W.

AU - Hernandez, Evelyn

AU - Miao, Jennifer

AU - Zee, Chih Te

AU - Novikova, Irina V.

AU - Goldschmidt, Lukasz

AU - McFarlane, Heather T.

AU - Helguera, Gustavo F.

AU - Evans, James E.

AU - Sawaya, Michael R.

AU - Cascio, Duilio

AU - Eisenberg, David S.

AU - Gonen, Tamir

AU - Rodriguez, Jose A.

PY - 2018/2/1

Y1 - 2018/2/1

N2 - The atomic structure of the infectious, protease-resistant, β-sheet-rich and fibrillar mammalian prion remains unknown. Through the cryo-EM method MicroED, we reveal the sub-ångström-resolution structure of a protofibril formed by a wild-type segment from the β2-α2 loop of the bank vole prion protein. The structure of this protofibril reveals a stabilizing network of hydrogen bonds that link polar zippers within a sheet, producing motifs we have named 'polar clasps'.

AB - The atomic structure of the infectious, protease-resistant, β-sheet-rich and fibrillar mammalian prion remains unknown. Through the cryo-EM method MicroED, we reveal the sub-ångström-resolution structure of a protofibril formed by a wild-type segment from the β2-α2 loop of the bank vole prion protein. The structure of this protofibril reveals a stabilizing network of hydrogen bonds that link polar zippers within a sheet, producing motifs we have named 'polar clasps'.

UR - https://www.scopus.com/pages/publications/85042755602

U2 - 10.1038/s41594-017-0018-0

DO - 10.1038/s41594-017-0018-0

M3 - Article

C2 - 29335561

SN - 1545-9993

VL - 25

SP - 131

EP - 134

JO - Nature Structural and Molecular Biology

JF - Nature Structural and Molecular Biology

IS - 2

ER -

Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp

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