Three-dimensional reconstruction of large tissue volumes from scanning laser confocal microscopy

M. A. Slamani; A. Krol; J. Beaumont; R. L. Price; I. L. Coman; E. D. Lipson

doi:10.1117/12.536232

Three-dimensional reconstruction of large tissue volumes from scanning laser confocal microscopy

M. A. Slamani
, A. Krol
, J. Beaumont
, R. L. Price
, I. L. Coman
, E. D. Lipson

Computer Science

SUNY Oswego

ITT Corporation
State University of New York (SUNY)
SUNY Upstate Medical University
University of Southern California
Syracuse University
Ithaca College

Research output: Contribution to journal › Conference article › peer-review

2 Scopus citations

Abstract

Phase correlation is applied to the mosaicing of confocal scanning laser microscopy data. A large specimen (i.e., a murine heart) is cut into a number of individual sections with appropriate thickness. The sections are scanned horizontally and vertically to produce tiles of a 3D volume. Image processing based on phase correlation is used to rebuild the 3D volume and stitch the tiles together. Specifically, 2D registration of in-plane tiles and 3D alignment of optical slices within a given physical section are performed. The approach and performance are presented in this paper along with examples.

Original language	English
Pages (from-to)	1972-1979
Number of pages	8
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	5370 III
DOIs	https://doi.org/10.1117/12.536232
State	Published - 2004
Event	Progress in Biomedical Optics and Imaging - Medical Imaging 2004: Imaging Processing - San Diego, CA, United States Duration: Feb 16 2004 → Feb 19 2004

Keywords

Confocal scanning laser microscopy
Mosaic
Phase correlation

Access to Document

10.1117/12.536232

Cite this

@article{9569d36b34e54789bd150ebf43ba0694,

title = "Three-dimensional reconstruction of large tissue volumes from scanning laser confocal microscopy",

abstract = "Phase correlation is applied to the mosaicing of confocal scanning laser microscopy data. A large specimen (i.e., a murine heart) is cut into a number of individual sections with appropriate thickness. The sections are scanned horizontally and vertically to produce tiles of a 3D volume. Image processing based on phase correlation is used to rebuild the 3D volume and stitch the tiles together. Specifically, 2D registration of in-plane tiles and 3D alignment of optical slices within a given physical section are performed. The approach and performance are presented in this paper along with examples.",

keywords = "Confocal scanning laser microscopy, Mosaic, Phase correlation",

author = "Slamani, \{M. A.\} and A. Krol and J. Beaumont and Price, \{R. L.\} and Coman, \{I. L.\} and Lipson, \{E. D.\}",

year = "2004",

doi = "10.1117/12.536232",

language = "English",

volume = "5370 III",

pages = "1972--1979",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

note = "Progress in Biomedical Optics and Imaging - Medical Imaging 2004: Imaging Processing ; Conference date: 16-02-2004 Through 19-02-2004",

}

TY - JOUR

T1 - Three-dimensional reconstruction of large tissue volumes from scanning laser confocal microscopy

AU - Slamani, M. A.

AU - Krol, A.

AU - Beaumont, J.

AU - Price, R. L.

AU - Coman, I. L.

AU - Lipson, E. D.

PY - 2004

Y1 - 2004

N2 - Phase correlation is applied to the mosaicing of confocal scanning laser microscopy data. A large specimen (i.e., a murine heart) is cut into a number of individual sections with appropriate thickness. The sections are scanned horizontally and vertically to produce tiles of a 3D volume. Image processing based on phase correlation is used to rebuild the 3D volume and stitch the tiles together. Specifically, 2D registration of in-plane tiles and 3D alignment of optical slices within a given physical section are performed. The approach and performance are presented in this paper along with examples.

AB - Phase correlation is applied to the mosaicing of confocal scanning laser microscopy data. A large specimen (i.e., a murine heart) is cut into a number of individual sections with appropriate thickness. The sections are scanned horizontally and vertically to produce tiles of a 3D volume. Image processing based on phase correlation is used to rebuild the 3D volume and stitch the tiles together. Specifically, 2D registration of in-plane tiles and 3D alignment of optical slices within a given physical section are performed. The approach and performance are presented in this paper along with examples.

KW - Confocal scanning laser microscopy

KW - Mosaic

KW - Phase correlation

UR - https://www.scopus.com/pages/publications/5644248151

U2 - 10.1117/12.536232

DO - 10.1117/12.536232

M3 - Conference article

SN - 0277-786X

VL - 5370 III

SP - 1972

EP - 1979

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Progress in Biomedical Optics and Imaging - Medical Imaging 2004: Imaging Processing

Y2 - 16 February 2004 through 19 February 2004

ER -

Three-dimensional reconstruction of large tissue volumes from scanning laser confocal microscopy

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this