Abstract
Aim: To examine the expression of pancreatic duodenal homeobox-1 (PDX-1) transcription factor in human colorectal cancer. Methods: RT-PCR, Western blotting, and immunohistochemistry were performed to determine the expression pattern of transcription factor PDX-1 in primary colorectal tumor, hepatic metastasis, and benign colon tissue from a single patient. Results: The highest PDX-1 transcription levels were detected in the metastasis material. Lower levels of PDX-1 were found to be present in the primary tumor, while normal colon tissue failed to express detectable levels of PDX-1. Western blot data revealed a PDX-1 expression pattern identical to that of mRNA expression. Immunohistochemistry confirmed high metastasis PDX-1 expression, lower levels in the primary tumor, and the presence of only traces of PDX-1 in normal colon tissue. Conclusion: These data argue for fur ther evaluation of PDX-1 as a biomarker for colorectal cancer.
| Original language | English |
|---|---|
| Pages (from-to) | 5823-5826 |
| Number of pages | 4 |
| Journal | World Journal of Gastroenterology |
| Volume | 14 |
| Issue number | 38 |
| DOIs | |
| State | Published - Oct 14 2008 |
Keywords
- Colorectal cancer
- Diagnostics
- Pancreatic duodenal homeobox-1
- Transcription factor
- Tumor marker
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