Transcriptional stimulation by CaPO4-DNA precipitates

Richard Pine; David E. Levy; Nancy Reich; James E. Darnell

doi:10.1093/nar/16.4.1371

Transcriptional stimulation by CaPO₄-DNA precipitates

Richard Pine
, David E. Levy
, Nancy Reich
, James E. Darnell

Microbiology and Immunology

Stony Brook University

Rockefeller University

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Genes in human chromosomes that normally require induction by α-interferon are activated after calcium phospate (CaPO₄) transfection, but not after DEAE-dextran transfection. The c-fos gene and genes stimulated by γ-interferon also are affected by CaPO₄-DNA precipitates, but the calcium ionophore A23187 stimulates only c-fos among this group. These results suggest caution not only in choosing gene transfer methods, but also in interpreting experiments aimed at understanding the role of second messengers in gene activation.

Original language	English
Pages (from-to)	1371-1378
Number of pages	8
Journal	Nucleic Acids Research
Volume	16
Issue number	4
DOIs	https://doi.org/10.1093/nar/16.4.1371
State	Published - Feb 25 1988

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title = "Transcriptional stimulation by CaPO4-DNA precipitates",

abstract = "Genes in human chromosomes that normally require induction by α-interferon are activated after calcium phospate (CaPO4) transfection, but not after DEAE-dextran transfection. The c-fos gene and genes stimulated by γ-interferon also are affected by CaPO4-DNA precipitates, but the calcium ionophore A23187 stimulates only c-fos among this group. These results suggest caution not only in choosing gene transfer methods, but also in interpreting experiments aimed at understanding the role of second messengers in gene activation.",

author = "Richard Pine and Levy, \{David E.\} and Nancy Reich and Darnell, \{James E.\}",

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T1 - Transcriptional stimulation by CaPO4-DNA precipitates

AU - Pine, Richard

AU - Levy, David E.

AU - Reich, Nancy

AU - Darnell, James E.

PY - 1988/2/25

Y1 - 1988/2/25

N2 - Genes in human chromosomes that normally require induction by α-interferon are activated after calcium phospate (CaPO4) transfection, but not after DEAE-dextran transfection. The c-fos gene and genes stimulated by γ-interferon also are affected by CaPO4-DNA precipitates, but the calcium ionophore A23187 stimulates only c-fos among this group. These results suggest caution not only in choosing gene transfer methods, but also in interpreting experiments aimed at understanding the role of second messengers in gene activation.

AB - Genes in human chromosomes that normally require induction by α-interferon are activated after calcium phospate (CaPO4) transfection, but not after DEAE-dextran transfection. The c-fos gene and genes stimulated by γ-interferon also are affected by CaPO4-DNA precipitates, but the calcium ionophore A23187 stimulates only c-fos among this group. These results suggest caution not only in choosing gene transfer methods, but also in interpreting experiments aimed at understanding the role of second messengers in gene activation.

UR - https://www.scopus.com/pages/publications/0024296267

U2 - 10.1093/nar/16.4.1371

DO - 10.1093/nar/16.4.1371

M3 - Article

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SN - 0305-1048

VL - 16

SP - 1371

EP - 1378

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 4

ER -

Transcriptional stimulation by CaPO₄-DNA precipitates

Abstract

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