Triplex formation-mediated fluorescence sensing of single bulge nucleotide-containing double-stranded RNAs using thiazole orange base surrogate-carrying PNAs

Tianxue Han; Yusuke Sato; Eriks Rozners; Seiichi Nishizawa

doi:10.1039/d5cc02960k

Triplex formation-mediated fluorescence sensing of single bulge nucleotide-containing double-stranded RNAs using thiazole orange base surrogate-carrying PNAs

Tianxue Han
, Yusuke Sato
, Eriks Rozners
, Seiichi Nishizawa

Chemistry

Binghamton University

Tohoku University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Fluorescence sensing of single bulge-containing double-stranded RNAs was achieved using a triplex forming peptide nucleic acid (PNA) carrying a thiazole orange (TO) base surrogate, where the TO base surrogate facing the bulged nucleotide exhibited a light-up response through triplex formation between the PNA sequence and target purine tracts in dsRNA.

Original language	English
Pages (from-to)	11778-11781
Number of pages	4
Journal	Chemical Communications
Volume	61
Issue number	63
DOIs	https://doi.org/10.1039/d5cc02960k
State	Published - Jul 31 2025

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title = "Triplex formation-mediated fluorescence sensing of single bulge nucleotide-containing double-stranded RNAs using thiazole orange base surrogate-carrying PNAs",

abstract = "Fluorescence sensing of single bulge-containing double-stranded RNAs was achieved using a triplex forming peptide nucleic acid (PNA) carrying a thiazole orange (TO) base surrogate, where the TO base surrogate facing the bulged nucleotide exhibited a light-up response through triplex formation between the PNA sequence and target purine tracts in dsRNA.",

author = "Tianxue Han and Yusuke Sato and Eriks Rozners and Seiichi Nishizawa",

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AU - Han, Tianxue

AU - Sato, Yusuke

AU - Rozners, Eriks

AU - Nishizawa, Seiichi

PY - 2025/7/31

Y1 - 2025/7/31

N2 - Fluorescence sensing of single bulge-containing double-stranded RNAs was achieved using a triplex forming peptide nucleic acid (PNA) carrying a thiazole orange (TO) base surrogate, where the TO base surrogate facing the bulged nucleotide exhibited a light-up response through triplex formation between the PNA sequence and target purine tracts in dsRNA.

AB - Fluorescence sensing of single bulge-containing double-stranded RNAs was achieved using a triplex forming peptide nucleic acid (PNA) carrying a thiazole orange (TO) base surrogate, where the TO base surrogate facing the bulged nucleotide exhibited a light-up response through triplex formation between the PNA sequence and target purine tracts in dsRNA.

UR - https://www.scopus.com/pages/publications/105009763534

U2 - 10.1039/d5cc02960k

DO - 10.1039/d5cc02960k

M3 - Article

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EP - 11781

JO - Chemical Communications

JF - Chemical Communications

IS - 63

ER -

Triplex formation-mediated fluorescence sensing of single bulge nucleotide-containing double-stranded RNAs using thiazole orange base surrogate-carrying PNAs

Abstract

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