Two step procedure for purification of enzymatically active prostate-specific antigen from seminal plasma

B. Bindukumar; Elzbieta Kawinski; Craig Cherrin; Leah M. Gambino; Madhavan P.N. Nair; Stanley A. Schwartz; Kailash C. Chadha

doi:10.1016/j.jchromb.2004.09.014

Two step procedure for purification of enzymatically active prostate-specific antigen from seminal plasma

B. Bindukumar
, Elzbieta Kawinski
, Craig Cherrin
, Leah M. Gambino
, Madhavan P.N. Nair
, Stanley A. Schwartz
, Kailash C. Chadha

Medicine

University at Buffalo

Roswell Park Cancer Institute
State University of New York (SUNY)

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The role of prostate-specific antigen (PSA) during the onset of prostate cancer and subsequent tumor growth and metastasis is not well understood. We have developed a simple two step procedure, based on principles of hydrophobic charge-induction chromatography and molecular size chromatography to provide pure free-PSA (f-PSA) preparation that is free from all other known PSA complexes as well as human kallikrein 2 (hK2). The overall recovery of f-PSA is 72%. The isolated f-PSA consists of three known isoforms that corresponds to pI of 6.2, 6.4 and 7.2. f-PSA is enzymatically active and its enzymatic activity can be effectively neutralized by a serine protease inhibitor.

Original language	English
Pages (from-to)	113-120
Number of pages	8
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	813
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jchromb.2004.09.014
State	Published - Dec 25 2004

Keywords

PSA
PSA purification
Prostate cancer
Prostate-specific antigen
Serine protease
Thiophilic-interaction chromatography

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10.1016/j.jchromb.2004.09.014

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Bindukumar, B., Kawinski, E., Cherrin, C., Gambino, L. M., Nair, M. P. N., Schwartz, S. A., & Chadha, K. C. (2004). Two step procedure for purification of enzymatically active prostate-specific antigen from seminal plasma. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 813(1-2), 113-120. https://doi.org/10.1016/j.jchromb.2004.09.014

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title = "Two step procedure for purification of enzymatically active prostate-specific antigen from seminal plasma",

abstract = "The role of prostate-specific antigen (PSA) during the onset of prostate cancer and subsequent tumor growth and metastasis is not well understood. We have developed a simple two step procedure, based on principles of hydrophobic charge-induction chromatography and molecular size chromatography to provide pure free-PSA (f-PSA) preparation that is free from all other known PSA complexes as well as human kallikrein 2 (hK2). The overall recovery of f-PSA is 72\%. The isolated f-PSA consists of three known isoforms that corresponds to pI of 6.2, 6.4 and 7.2. f-PSA is enzymatically active and its enzymatic activity can be effectively neutralized by a serine protease inhibitor.",

keywords = "PSA, PSA purification, Prostate cancer, Prostate-specific antigen, Serine protease, Thiophilic-interaction chromatography",

author = "B. Bindukumar and Elzbieta Kawinski and Craig Cherrin and Gambino, \{Leah M.\} and Nair, \{Madhavan P.N.\} and Schwartz, \{Stanley A.\} and Chadha, \{Kailash C.\}",

year = "2004",

month = dec,

day = "25",

doi = "10.1016/j.jchromb.2004.09.014",

language = "English",

volume = "813",

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journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

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Bindukumar, B, Kawinski, E, Cherrin, C, Gambino, LM, Nair, MPN, Schwartz, SA & Chadha, KC 2004, 'Two step procedure for purification of enzymatically active prostate-specific antigen from seminal plasma', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 813, no. 1-2, pp. 113-120. https://doi.org/10.1016/j.jchromb.2004.09.014

Two step procedure for purification of enzymatically active prostate-specific antigen from seminal plasma. / Bindukumar, B.; Kawinski, Elzbieta; Cherrin, Craig et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 813, No. 1-2, 25.12.2004, p. 113-120.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Two step procedure for purification of enzymatically active prostate-specific antigen from seminal plasma

AU - Bindukumar, B.

AU - Kawinski, Elzbieta

AU - Cherrin, Craig

AU - Gambino, Leah M.

AU - Nair, Madhavan P.N.

AU - Schwartz, Stanley A.

AU - Chadha, Kailash C.

PY - 2004/12/25

Y1 - 2004/12/25

N2 - The role of prostate-specific antigen (PSA) during the onset of prostate cancer and subsequent tumor growth and metastasis is not well understood. We have developed a simple two step procedure, based on principles of hydrophobic charge-induction chromatography and molecular size chromatography to provide pure free-PSA (f-PSA) preparation that is free from all other known PSA complexes as well as human kallikrein 2 (hK2). The overall recovery of f-PSA is 72%. The isolated f-PSA consists of three known isoforms that corresponds to pI of 6.2, 6.4 and 7.2. f-PSA is enzymatically active and its enzymatic activity can be effectively neutralized by a serine protease inhibitor.

AB - The role of prostate-specific antigen (PSA) during the onset of prostate cancer and subsequent tumor growth and metastasis is not well understood. We have developed a simple two step procedure, based on principles of hydrophobic charge-induction chromatography and molecular size chromatography to provide pure free-PSA (f-PSA) preparation that is free from all other known PSA complexes as well as human kallikrein 2 (hK2). The overall recovery of f-PSA is 72%. The isolated f-PSA consists of three known isoforms that corresponds to pI of 6.2, 6.4 and 7.2. f-PSA is enzymatically active and its enzymatic activity can be effectively neutralized by a serine protease inhibitor.

KW - PSA

KW - PSA purification

KW - Prostate cancer

KW - Prostate-specific antigen

KW - Serine protease

KW - Thiophilic-interaction chromatography

UR - https://www.scopus.com/pages/publications/8844244025

U2 - 10.1016/j.jchromb.2004.09.014

DO - 10.1016/j.jchromb.2004.09.014

M3 - Article

C2 - 15556523

SN - 1570-0232

VL - 813

SP - 113

EP - 120

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 1-2

ER -

Two step procedure for purification of enzymatically active prostate-specific antigen from seminal plasma

Abstract

Keywords

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