Abstract
Capillary liquid chromatography at moderately high pressures and capillary electrochromatography (CEC) have been combined to drive the mobile phase through capillary columns packed with small diameter particles. In a column packed with 1.5 μm nonporous particles, linear velocities near 3 mm/s were observed when combining inlet pressures of 690 bar (10,000 psi) and an applied voltage of 25 kV. Optimum linear velocity for the column was achieved using a pressure-voltage combination of 350 bar (5000 psi) and 5 kV. Separation efficiencies at near optimum linear velocity agreed with those predicted by the van Deemter equation for liquid chromatography. Retention factors were observed to decrease under pressure-voltage combination as the voltage was increased; such a behavior has been attributed to Joule heating effects.
| Original language | English |
|---|---|
| Pages (from-to) | 131-138 |
| Number of pages | 8 |
| Journal | Journal of Chromatography A |
| Volume | 1106 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Feb 17 2006 |
Keywords
- Capillary electrochromatography
- Liquid chromatography
- Pressure assisted capillary electrochromatography
- Voltage-assisted capillary liquid chromatography
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