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ViPRE: An open-source software implementation for end-to-end analysis of mass spectrometry data

  • H. Elmi
  • , A. Jamzad
  • , M. Sharp
  • , J. R. Rodgers
  • , M. Kaufmann
  • , T. Jamaspishvili
  • , R. Iseman
  • , D. Berman
  • , J. Rudan
  • , G. Fichtinger
  • , P. Mousavi
    • Queen's University Kingston
    • University of Manitoba

    Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

    Abstract

    Mass spectrometry imaging (MSI) is a powerful tool capable of visualizing molecular patterns to identify disease markers in tissue analysis. However, data analysis is computationally heavy and currently time-consuming as there is no single platform capable of performing the entire preprocessing, visualization, and analysis pipeline end-to-end. Using different software tools and file formats required for such tools also makes the process prone to error. The purpose of this work is to develop a free, open-source software implementation called “Visualization, Preprocessing, and Registration Environment” (ViPRE), capable of end-to-end analysis of MSI data. ViPRE was developed to provide various functionalities required for MSI analysis including data import, data visualization, data registration, Region of Interest (ROI) selection, spectral data alignment and data analysis. The software implementation is offered as an open-source module in 3D Slicer, a medical imaging platform. It is also designed for flexibility and usability throughout the user experience. ViPRE was tested using sample MSI data to evaluate the computational pipeline, with the results showing successful implementation of its functionalities and end-to-end usage. A preliminary usability test was also performed to assess user experience, with findings showing positive results. ViPRE aspires to satisfy the need for a single-stop comprehensive interface for MSI data analysis. The source code and documentation will be made publicly available.

    Original languageEnglish
    Title of host publicationMedical Imaging 2023
    Subtitle of host publicationImage-Guided Procedures, Robotic Interventions, and Modeling
    EditorsCristian A. Linte, Jeffrey H. Siewerdsen
    PublisherSPIE
    ISBN (Electronic)9781510660373
    DOIs
    StatePublished - 2023
    EventMedical Imaging 2023: Image-Guided Procedures, Robotic Interventions, and Modeling - San Diego, United States
    Duration: Feb 19 2023Feb 23 2023

    Publication series

    NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
    Volume12466

    Conference

    ConferenceMedical Imaging 2023: Image-Guided Procedures, Robotic Interventions, and Modeling
    Country/TerritoryUnited States
    CitySan Diego
    Period02/19/2302/23/23

    Keywords

    • Mass spectrometry imaging (MSI)
    • desorption electrospray ionization (DESI)
    • end-to-end software implementation

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