Vitamin D status modulates rat colonic M3 muscarinic receptor characteristics and coupling to guanylate cyclase

Sharad Khare; Xiao Ying Tien; David Wilson; Ramesh K. Wali; Bruce M. Bissonnette; Michael D. Sitrin; Thomas A. Brasitus

doi:10.1016/0014-5793(94)00498-6

Vitamin D status modulates rat colonic M₃ muscarinic receptor characteristics and coupling to guanylate cyclase

Sharad Khare
, Xiao Ying Tien
, David Wilson
, Ramesh K. Wali
, Bruce M. Bissonnette
, Michael D. Sitrin
, Thomas A. Brasitus

Medicine

University at Buffalo

The University of Chicago

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The present studies were conducted to determine whether [³H]quinuclidinyl benzilate binding in rat colonic membranes and/or carbachol-mediated stimulation of particulate guanylate cyclase were altered by changes in vitamin D status. EC₅₀ values for the stimulation of colonic guanylate cyclase by carbachol were found to be significantly greater in vitamin D-deficient rats compared to their D-sufficient counterparts. Concomitantly, the density of receptors (B_max) were significantly lower, and dissociation constants (K_d) were significantly higher in D-deficient colonic membranes. In vitamin D-repleted animals, moreover, all of these aforementioned alterations were at least partially corrected.

Original language	English
Pages (from-to)	27-30
Number of pages	4
Journal	FEBS Letters
Volume	347
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(94)00498-6
State	Published - Jun 20 1994

Keywords

Cyclic GMP
Cyclic nucleotide
Muscarinic agonist
Polyphosphoinositide
Rat large intestine
Signal transduction

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10.1016/0014-5793(94)00498-6

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title = "Vitamin D status modulates rat colonic M3 muscarinic receptor characteristics and coupling to guanylate cyclase",

abstract = "The present studies were conducted to determine whether [3H]quinuclidinyl benzilate binding in rat colonic membranes and/or carbachol-mediated stimulation of particulate guanylate cyclase were altered by changes in vitamin D status. EC50 values for the stimulation of colonic guanylate cyclase by carbachol were found to be significantly greater in vitamin D-deficient rats compared to their D-sufficient counterparts. Concomitantly, the density of receptors (Bmax) were significantly lower, and dissociation constants (Kd) were significantly higher in D-deficient colonic membranes. In vitamin D-repleted animals, moreover, all of these aforementioned alterations were at least partially corrected.",

keywords = "Cyclic GMP, Cyclic nucleotide, Muscarinic agonist, Polyphosphoinositide, Rat large intestine, Signal transduction",

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AU - Khare, Sharad

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AU - Wilson, David

AU - Wali, Ramesh K.

AU - Bissonnette, Bruce M.

AU - Sitrin, Michael D.

AU - Brasitus, Thomas A.

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N2 - The present studies were conducted to determine whether [3H]quinuclidinyl benzilate binding in rat colonic membranes and/or carbachol-mediated stimulation of particulate guanylate cyclase were altered by changes in vitamin D status. EC50 values for the stimulation of colonic guanylate cyclase by carbachol were found to be significantly greater in vitamin D-deficient rats compared to their D-sufficient counterparts. Concomitantly, the density of receptors (Bmax) were significantly lower, and dissociation constants (Kd) were significantly higher in D-deficient colonic membranes. In vitamin D-repleted animals, moreover, all of these aforementioned alterations were at least partially corrected.

AB - The present studies were conducted to determine whether [3H]quinuclidinyl benzilate binding in rat colonic membranes and/or carbachol-mediated stimulation of particulate guanylate cyclase were altered by changes in vitamin D status. EC50 values for the stimulation of colonic guanylate cyclase by carbachol were found to be significantly greater in vitamin D-deficient rats compared to their D-sufficient counterparts. Concomitantly, the density of receptors (Bmax) were significantly lower, and dissociation constants (Kd) were significantly higher in D-deficient colonic membranes. In vitamin D-repleted animals, moreover, all of these aforementioned alterations were at least partially corrected.

KW - Cyclic GMP

KW - Cyclic nucleotide

KW - Muscarinic agonist

KW - Polyphosphoinositide

KW - Rat large intestine

KW - Signal transduction

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EP - 30

JO - FEBS Letters

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ER -

Vitamin D status modulates rat colonic M₃ muscarinic receptor characteristics and coupling to guanylate cyclase

Abstract

Keywords

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