Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain

Dhanalakshmi Nair; Guido Memmi; David Hernandez; Jonathan Bard; Marie Beaume; Steven Gill; Patrice Francois; Ambrose L. Cheung

doi:10.1128/JB.00027-11

Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain

Dhanalakshmi Nair
, Guido Memmi
, David Hernandez
, Jonathan Bard
, Marie Beaume
, Steven Gill
, Patrice Francois
, Ambrose L. Cheung

Biochemistry

University at Buffalo

Research output: Contribution to journal › Article › peer-review

166 Scopus citations

Abstract

Staphylococcus aureus RN4220, a cloning intermediate, is sometimes used in virulence, resistance, and metabolic studies. Using whole-genome sequencing, we showed that RN4220 differs from NCTC8325 and contains a number of genetic polymorphisms that affect both virulence and general fitness, implying a need for caution in using this strain for such studies.

Original language	English
Pages (from-to)	2332-2335
Number of pages	4
Journal	Journal of Bacteriology
Volume	193
Issue number	9
DOIs	https://doi.org/10.1128/JB.00027-11
State	Published - May 2011

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Nair, D., Memmi, G., Hernandez, D., Bard, J., Beaume, M., Gill, S., Francois, P., & Cheung, A. L. (2011). Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain. Journal of Bacteriology, 193(9), 2332-2335. https://doi.org/10.1128/JB.00027-11

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title = "Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain",

abstract = "Staphylococcus aureus RN4220, a cloning intermediate, is sometimes used in virulence, resistance, and metabolic studies. Using whole-genome sequencing, we showed that RN4220 differs from NCTC8325 and contains a number of genetic polymorphisms that affect both virulence and general fitness, implying a need for caution in using this strain for such studies.",

author = "Dhanalakshmi Nair and Guido Memmi and David Hernandez and Jonathan Bard and Marie Beaume and Steven Gill and Patrice Francois and Cheung, \{Ambrose L.\}",

year = "2011",

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doi = "10.1128/JB.00027-11",

language = "English",

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pages = "2332--2335",

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Nair, D, Memmi, G, Hernandez, D, Bard, J, Beaume, M, Gill, S, Francois, P & Cheung, AL 2011, 'Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain', Journal of Bacteriology, vol. 193, no. 9, pp. 2332-2335. https://doi.org/10.1128/JB.00027-11

Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain. / Nair, Dhanalakshmi; Memmi, Guido; Hernandez, David et al.
In: Journal of Bacteriology, Vol. 193, No. 9, 05.2011, p. 2332-2335.

Research output: Contribution to journal › Article › peer-review

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AU - Nair, Dhanalakshmi

AU - Memmi, Guido

AU - Hernandez, David

AU - Bard, Jonathan

AU - Beaume, Marie

AU - Gill, Steven

AU - Francois, Patrice

AU - Cheung, Ambrose L.

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AB - Staphylococcus aureus RN4220, a cloning intermediate, is sometimes used in virulence, resistance, and metabolic studies. Using whole-genome sequencing, we showed that RN4220 differs from NCTC8325 and contains a number of genetic polymorphisms that affect both virulence and general fitness, implying a need for caution in using this strain for such studies.

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Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain

Abstract

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